10+ Ezyhaul Interview Questions and Answers

Flow through collection is a process of continuous sample collection while fraction collection is the process of collecting specific portions of a sample.

• Flow through collection involves collecting a continuous stream of sample without interruption

• Fraction collection involves collecting specific portions of a sample based on predetermined criteria

• Both processes are commonly used in chromatography and other analytical techniques

• Flow through collection is useful for obtaining a...read more

The molecular weight of the target protein is unknown.

• The molecular weight of a protein is the sum of the atomic weights of all the atoms in the protein molecule.

• It is an important parameter for protein purification and characterization.

• The molecular weight can be determined using techniques such as mass spectrometry or gel electrophoresis.

The overall pH and conductivity of the DS depend on the specific DS being referred to.

• The pH and conductivity of different DS can vary widely depending on their composition and intended use.

• For example, the pH of a cleaning solution DS may be around 10-11, while the pH of a battery electrolyte DS may be around 1-2.

• Similarly, the conductivity of a DS used in electronics manufacturing may be very low, while the conductivity of a DS used in metal plating may be very high.

CEX Fraction pooling is done based on the desired purity and yield of the final product.

• CEX Fraction pooling is a process used in protein purification.

• It involves combining multiple fractions from a cation exchange chromatography column.

• The fractions are pooled based on the desired purity and yield of the final product.

• Factors such as protein concentration, pH, and buffer composition are also considered.

• CEX Fraction pooling can help improve the overall efficiency and yield of...read more

Ultrafiltration is a process of separating molecules based on their size using a semipermeable membrane. Diafiltration is a process of washing the separated molecules to remove impurities.

• Ultrafiltration is used in industries such as food and beverage, pharmaceuticals, and biotechnology to purify and concentrate solutions.

• Diafiltration is commonly used in protein purification to remove salts and other impurities from the solution.

• Both ultrafiltration and diafiltration are imp...read more

Protein binding to resin is a process where proteins are immobilized onto a solid support matrix.

• Resin is typically made of a porous material such as agarose or cellulose beads

• Proteins bind to the resin through various interactions such as hydrogen bonding, electrostatic interactions, and hydrophobic interactions

• This process is commonly used in protein purification and chromatography techniques

• The choice of resin and binding conditions depend on the specific protein being pur...read more

Isoelectric point is the pH at which a molecule has no net charge.

• It is the pH at which a molecule has equal number of positive and negative charges.

• It is also known as the pI.

• At the isoelectric point, a molecule will not migrate in an electric field.

• It is important in protein purification and separation techniques.

AWFI can be qualified through testing and certification processes.

• AWFI can be qualified through third-party testing and certification processes

• Testing may include performance, safety, and environmental standards

• Certification may be required by regulatory bodies or industry associations

• Examples of AWFI certification programs include UL, CE, and ISO

• Qualification may also involve audits of manufacturing processes and quality control measures

NWP stands for Normalized Water Permeability in Tangential Flow Filtration (TFF).

• NWP is a measure of the water permeability of a TFF membrane normalized by its surface area and thickness.

• It is used to evaluate the performance of TFF membranes in terms of their ability to retain solutes while allowing water to pass through.

• Higher NWP values indicate better membrane performance.

• NWP is affected by factors such as membrane material, pore size, and operating conditions.

• For example...read more