Serum Institute of India Manufacturing Executive Interview Questions and Answers

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Q1. Buy and Sell Stock
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Q1. Explain process flow
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Process flow is the sequence of steps involved in the production of a product or service.
• Process flow outlines the steps required to complete a task or project

• It helps to identify bottlenecks and areas for improvement

• Examples include manufacturing processes, software development, and customer service workflows
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Q2. Principle of protein binding to resin
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Protein binding to resin is a process where proteins are immobilized onto a solid support matrix.
• Resin is typically made of a porous material such as agarose or cellulose beads

• Proteins bind to the resin through various interactions such as hydrogen bonding, electrostatic interactions, and hydrophobic interactions

• This process is commonly used in protein purification and chromatography techniques

• The choice of resin and b...read more
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Q3. What is isoelectric point
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Isoelectric point is the pH at which a molecule has no net charge.
• It is the pH at which a molecule has equal number of positive and negative charges.

• It is also known as the pI.

• At the isoelectric point, a molecule will not migrate in an electric field.

• It is important in protein purification and separation techniques.
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Q4. What is Ultrafiltration and diafiltration
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Ultrafiltration is a process of separating molecules based on their size using a semipermeable membrane. Diafiltration is a process of washing the separated molecules to remove impurities.
• Ultrafiltration is used in industries such as food and beverage, pharmaceuticals, and biotechnology to purify and concentrate solutions.

• Diafiltration is commonly used in protein purification to remove salts and other impurities from t...read more
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Q5. How do we qualify AWFI
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AWFI can be qualified through testing and certification processes.
• AWFI can be qualified through third-party testing and certification processes

• Testing may include performance, safety, and environmental standards

• Certification may be required by regulatory bodies or industry associations

• Examples of AWFI certification programs include UL, CE, and ISO

• Qualification may also involve audits of manufacturing processes and qual
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Q6. What is flow through collection and fraction collection
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Flow through collection is a process of continuous sample collection while fraction collection is the process of collecting specific portions of a sample.
• Flow through collection involves collecting a continuous stream of sample without interruption

• Fraction collection involves collecting specific portions of a sample based on predetermined criteria

• Both processes are commonly used in chromatography and other analytical t...read more
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Q7. On what basis CEX Fraction pooling is done
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CEX Fraction pooling is done based on the desired purity and yield of the final product.
• CEX Fraction pooling is a process used in protein purification.

• It involves combining multiple fractions from a cation exchange chromatography column.

• The fractions are pooled based on the desired purity and yield of the final product.

• Factors such as protein concentration, pH, and buffer composition are also considered.

• CEX Fraction po...read more
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Q8. What is the overall pH and conductivity of the DS
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The overall pH and conductivity of the DS depend on the specific DS being referred to.
• The pH and conductivity of different DS can vary widely depending on their composition and intended use.

• For example, the pH of a cleaning solution DS may be around 10-11, while the pH of a battery electrolyte DS may be around 1-2.

• Similarly, the conductivity of a DS used in electronics manufacturing may be very low, while the conductiv...read more
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Q9. What is the molecular weight of the target protein
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The molecular weight of the target protein is unknown.
• The molecular weight of a protein is the sum of the atomic weights of all the atoms in the protein molecule.

• It is an important parameter for protein purification and characterization.

• The molecular weight can be determined using techniques such as mass spectrometry or gel electrophoresis.
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Q10. What is NWP in TFF
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NWP stands for Normalized Water Permeability in Tangential Flow Filtration (TFF).
• NWP is a measure of the water permeability of a TFF membrane normalized by its surface area and thickness.

• It is used to evaluate the performance of TFF membranes in terms of their ability to retain solutes while allowing water to pass through.

• Higher NWP values indicate better membrane performance.

• NWP is affected by factors such as membrane...read more
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