Serum Institute of India Assistant Manager/Manager -(Technical) Interview Questions and Answers

Passaging cells involves detaching cells from a culture vessel, diluting them, and transferring them to a new vessel to allow for continued growth.

• Use a sterile technique to detach cells from the culture vessel

• Collect the cells in a centrifuge tube and centrifuge to pellet the cells

• Resuspend the cells in fresh media and calculate the appropriate dilution factor

• Transfer the diluted cells to a new culture vessel and incu

Protein structure refers to the three-dimensional arrangement of atoms in a protein molecule, which determines its function.

• Proteins are made up of amino acids, which are linked together by peptide bonds.

• The primary structure of a protein is the linear sequence of amino acids.

• The secondary structure refers to local folding patterns, such as alpha helices and beta sheets.

• The tertiary structure is the overall three-dimen...read more