Biocon Biologics Executive Interview Questions and Answers

Q1. Explain the process from vial thaw
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Q2. Available to work in shift
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Q1. Why flow-through is collected during AEX chromatography?
Ans. 
Flow-through is collected during AEX chromatography to separate unbound molecules from the target molecule bound to the resin.
• Flow-through contains molecules that did not bind to the resin

• Target molecule is retained on the resin due to interactions with the stationary phase

• Flow-through is collected to isolate the target molecule for further analysis
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Q2. What is Ion Exchange Chromatography?
Ans. 
Ion exchange chromatography is a technique used to separate and purify proteins and other charged molecules based on their net charge.
• Separates molecules based on their charge

• Uses a stationary phase with charged groups to attract and bind charged molecules

• Elution is achieved by changing the ionic strength or pH of the mobile phase

• Commonly used in protein purification and analysis
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Had 4 different sections

Q1. Cleaning room for activity, crystalline structure activities
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Q2. Cleaning room for meaning that is separate dedicated high cleaning improvement classified area , this area responsible stable on AHU FOR laminar air conditioning and outer microbio avoid practicals and dif...read more
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Q1. Company accordingly
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Q1. All about the process
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Q2. All about the previous experience
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