Swati Spentose Interview Questions and Answers

The parameter of HPLC depends on the type of analysis being performed.

• The choice of column and mobile phase affects the separation of analytes

• Retention time and peak area are commonly used parameters

• Resolution, selectivity, and efficiency are also important parameters

• Different parameters may be more important for different types of analysis

• For example, quantification may require a different parameter than identificatio

The parameter of Gas Chromatography refers to the variable being measured or analyzed in the process.

• The parameter can be the retention time of the analyte, the peak area, or the peak height.

• It can also be the resolution between two peaks or the selectivity factor.

• The parameter is important in determining the identity and quantity of the analyte in the sample.

• It can also be used to optimize the separation conditions fo

Peak shape in melting range can be broad or sharp depending on factors like sample purity, heating rate, and instrument calibration.

• Broad peak shape can indicate impurities or a mixture of compounds in the sample.

• Sharp peak shape suggests a pure compound with a narrow melting range.

• Heating rate affects peak shape, with slower rates often resulting in sharper peaks.

• Instrument calibration and resolution can also impact p...read more

The columns used during compositional accuracy of HPLC calibration are known as reference standards.

• Reference standards are used to ensure the accuracy and precision of HPLC calibration.

• These standards are typically composed of known compounds that are used to calibrate the HPLC system.

• The reference standards are run through the HPLC system and the resulting data is compared to the expected values.

• If the results are wi...read more

Caffeine is used in HPLC calibration as a reference compound due to its unique properties.

• Caffeine has a well-defined retention time and UV absorbance spectrum, making it a reliable reference compound.

• It is also non-toxic and easily available, making it a convenient choice for calibration.

• Caffeine is used to determine the retention time, peak shape, and resolution of the HPLC system.

• It is also used to check the accurac...read more

Acetone is used in HPLC calibration as a solvent and to remove impurities.

• Acetone is a common solvent used in HPLC calibration

• It is used to dissolve the sample and standards

• Acetone is also used to remove impurities from the HPLC system

• It is a volatile solvent and evaporates quickly

• Acetone is compatible with most HPLC columns and detectors