Swati Spentose QC Manager Interview Questions, Process, and Tips

Peak shape in melting range can be broad or sharp depending on factors like sample purity, heating rate, and instrument calibration.

• Broad peak shape can indicate impurities or a mixture of compounds in the sample.

• Sharp peak shape suggests a pure compound with a narrow melting range.

• Heating rate affects peak shape, with slower rates often resulting in sharper peaks.

• Instrument calibration and resolution can also impact p...read more

The columns used during compositional accuracy of HPLC calibration are known as reference standards.

• Reference standards are used to ensure the accuracy and precision of HPLC calibration.

• These standards are typically composed of known compounds that are used to calibrate the HPLC system.

• The reference standards are run through the HPLC system and the resulting data is compared to the expected values.

• If the results are wi...read more

Caffeine is used in HPLC calibration as a reference compound due to its unique properties.

• Caffeine has a well-defined retention time and UV absorbance spectrum, making it a reliable reference compound.

• It is also non-toxic and easily available, making it a convenient choice for calibration.

• Caffeine is used to determine the retention time, peak shape, and resolution of the HPLC system.

• It is also used to check the accurac...read more

Acetone is used in HPLC calibration as a solvent and to remove impurities.

• Acetone is a common solvent used in HPLC calibration

• It is used to dissolve the sample and standards

• Acetone is also used to remove impurities from the HPLC system

• It is a volatile solvent and evaporates quickly

• Acetone is compatible with most HPLC columns and detectors