Institute of Forest Genetics and Tree Breeding Senior Project Fellow Interview Questions and Answers for Experienced

Bulk Segregation Analysis is a method used to identify genetic markers linked to a specific trait by analyzing the segregation of markers in a population.

• BSA involves pooling DNA samples from individuals with the trait of interest and comparing them to individuals without the trait.

• Microsatellite markers are used to genotype the pooled DNA samples and identify regions of the genome that are linked to the trait.

• By analy...read more

Bioinformatics tools are used to assess population genetic structure by analyzing genetic data from different populations.

• Population structure analysis tools include STRUCTURE, ADMIXTURE, and PCA

• These tools help identify genetic clusters, admixture, and relatedness among individuals

• They are used to study evolutionary relationships, migration patterns, and genetic diversity within populations

Tools like Primer3, BLAST, and SSRFinder are used to design microsatellite markers using genome and transcriptome data.

• Primer3 is used to design PCR primers for amplifying microsatellite regions

• BLAST is used to search for homologous sequences in the genome or transcriptome data

• SSRFinder is used to identify potential microsatellite regions in the sequences

• Other tools like MISA and QDD may also be used for microsatellite