Glenmark Pharmaceuticals Research Scientist Interview Questions and Answers

Mass spectrometry is a technique used to identify and quantify molecules based on their mass-to-charge ratio.

• Electrospray ionization (ESI)

• Matrix-assisted laser desorption/ionization (MALDI)

• Gas chromatography-mass spectrometry (GC-MS)

• Liquid chromatography-mass spectrometry (LC-MS)

• Time-of-flight mass spectrometry (TOF-MS)

UV spectrometry is a technique that measures the absorption or transmission of ultraviolet light by a sample.

• UV spectrometry is based on the principle that different molecules absorb UV light at specific wavelengths.

• It is commonly used in analytical chemistry to determine the concentration of a substance in a sample.

• UV spectrometry can be used to analyze the purity of a compound or identify unknown substances.

• The instr...read more

To prepare 0.1 N HCl and NaOH, you need to calculate the required amount of the respective chemicals and dilute them in water.

• Calculate the molar mass of HCl and NaOH

• Determine the volume of solution needed

• Use the formula: Normality (N) = (Number of equivalents / Volume of solution in liters)

• For 0.1 N HCl, calculate the number of equivalents of HCl and dilute it to the desired volume

• For NaOH, calculate the number of equ...read more

Quality assurance is the process of ensuring that products or services meet the desired level of quality.

• It involves establishing standards and procedures to ensure that products or services meet those standards.

• It includes monitoring and evaluating the quality of products or services to identify areas for improvement.

• It also involves taking corrective action when necessary to address quality issues.

• Examples include co...read more

Recall definitions

• Recall: the ability to retrieve information from memory

• Definition: a statement that explains the meaning of a word or phrase

• Research: the systematic investigation into and study of materials and sources in order to establish facts and reach new conclusions

• Associate: a person who works with others towards a common goal

• Hypothesis: a proposed explanation for a phenomenon

• Experiment: a scientific procedure...read more

The type of HPLC used depends on the specific research requirements and objectives.

• There are several types of HPLC systems available, including reverse phase, normal phase, ion exchange, size exclusion, and affinity chromatography.

• The choice of HPLC system depends on the properties of the analyte, such as polarity, size, charge, and affinity.

• For example, reverse phase HPLC is commonly used for the separation of nonpola...read more

UV detectors and HPLC detectors are used in research to analyze and quantify substances.

• UV detectors are commonly used in UV-visible spectroscopy to measure the absorption of light by a sample at different wavelengths.

• HPLC detectors are used in high-performance liquid chromatography to detect and quantify the components of a mixture.

• Common types of UV detectors include diode array detectors (DAD) and photodiode array d...read more

Matrix in MALDI MS system is a substance used to assist in ionization of the sample for analysis.

• Matrix is a substance that absorbs energy from the laser and transfers it to the sample, causing ionization.

• The choice of matrix depends on the type of sample being analyzed.

• Common matrices include alpha-cyano-4-hydroxycinnamic acid (CHCA) and sinapinic acid (SA).

Sparging nitrogen during filling is done to prevent oxidation and maintain product quality.

• Sparging nitrogen displaces oxygen from the container, preventing oxidation of the product.

• Oxidation can lead to degradation of the product, affecting its quality and stability.

• Nitrogen is an inert gas that does not react with the product, ensuring its integrity.

• Sparging nitrogen is commonly used in the pharmaceutical and food in...read more

Analysis is done through a systematic process of collecting, organizing, and interpreting data.

• Data collection: Gather relevant information and data.

• Data organization: Arrange the collected data in a structured manner.

• Data interpretation: Analyze the data to draw meaningful conclusions.

• Use of statistical methods: Apply statistical techniques to analyze the data.

• Reporting: Present the findings and conclusions in a clear

Precautions taken during analysis

• Ensuring accurate and reliable data collection

• Maintaining proper documentation and record-keeping

• Following established protocols and procedures

• Implementing quality control measures

• Validating and verifying results

• Protecting confidentiality and privacy of data

• Considering potential biases and limitations

• Applying statistical analysis techniques appropriately

To safely handle instruments and equipment, proper storage, regular maintenance, and following safety protocols are essential.

• Store instruments and equipment in designated areas to prevent damage or loss.

• Clean and disinfect instruments after each use to maintain hygiene.

• Regularly inspect equipment for any signs of wear or malfunction.

• Follow safety guidelines and protocols while handling instruments to prevent accidents...read more

Seeking new challenges and opportunities for growth.

• Desire for new challenges and opportunities for growth

• Seeking a more stimulating work environment

• Looking to expand skill set and knowledge

• Company restructuring or downsizing

• Relocation to a new area

HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate, identify, and quantify components in a mixture.

• HPLC is a widely used analytical technique in chemistry and biochemistry.

• It involves the use of a liquid mobile phase and a solid stationary phase to separate the components of a mixture based on their interactions with the stationary phase.

• The components are separated based on thei...read more

Zetasizer measures particle size and zeta potential while Mastersizer measures particle size distribution.

• Zetasizer uses dynamic light scattering to measure particle size and zeta potential.

• Mastersizer uses laser diffraction to measure particle size distribution.

• Zetasizer is used for colloidal systems while Mastersizer is used for dry powders and granules.

• Zetasizer can measure particles in the range of 0.3 nm to 10 mic...read more

HPLC and UPLC are both chromatography techniques used for separation and analysis of compounds, but differ in their operating pressures and particle sizes.

• HPLC uses larger particle sizes and operates at lower pressures than UPLC.

• UPLC uses smaller particle sizes and operates at higher pressures than HPLC.

• UPLC provides higher resolution and faster separations than HPLC.

• HPLC is more commonly used for routine analysis, whi...read more