10+ Junior Scientist Interview Questions and Answers

Common contaminations in cell culture include bacterial, fungal, viral, and mycoplasma contaminations.

• Bacterial contamination: Evidenced by turbidity, pH changes, and presence of biofilms.

• Fungal contamination: Visible as fuzzy growth or discoloration in the culture.

• Viral contamination: Detected through cytopathic effects or specific viral assays.

• Mycoplasma contamination: Difficult to detect visually, requires PCR or DNA staining methods.

Yes, I have experience in primary tumor cell culture.

• I have worked with primary tumor cells in a laboratory setting.

• I am familiar with the techniques and protocols involved in primary tumor cell culture.

• I have successfully cultured primary tumor cells for various experiments and assays.

• I have experience in maintaining the viability and growth of primary tumor cells in culture.

• I am knowledgeable about the challenges and considerations specific to primary tumor cell culture.

To increase dissolution, one can use various techniques such as selecting appropriate diluents, binders, lubricants, disintegrants, and coatings.

• Diluent: An inert substance added to a drug formulation to increase its bulk and aid in the dissolution process. Example: lactose.

• Binder: A substance that helps in binding the ingredients of a tablet or capsule together. Example: hydroxypropyl cellulose.

• Lubrication: The process of reducing friction between particles to enhance dissol...read more

Yes, I know how to culture primary tumor cells.

• Primary tumor cells can be cultured by isolating them from the tumor tissue.

• The cells are then placed in a suitable culture medium that provides the necessary nutrients and growth factors.

• The culture conditions, such as temperature, humidity, and CO2 levels, are optimized to support cell growth.

• Regular monitoring and maintenance of the culture are required to ensure cell viability and prevent contamination.

• Primary tumor cells can...read more

During validation, parameters such as accuracy, precision, specificity, sensitivity, and reproducibility are performed.

• Accuracy - how close the measured value is to the true value

• Precision - how close the measured values are to each other

• Specificity - ability to correctly identify negative results

• Sensitivity - ability to correctly identify positive results

• Reproducibility - ability to obtain consistent results across multiple tests

Tablets and capsules are two common types of oral medication. Dissolution is the process of the medication breaking down in the body.

• Tablets are solid, compressed medication that come in various shapes and sizes.

• Capsules are medication enclosed in a gelatin shell.

• Dissolution is the process of the medication breaking down in the body and being absorbed into the bloodstream.

• The rate of dissolution can affect the medication's effectiveness and side effects.

• Factors that can affec...read more

The disintegrant test is used to determine the time taken for a tablet to disintegrate in a specified medium.

• Disintegrants are added to tablets to aid in their breakdown and dissolution in the body.

• The disintegrant test is performed using a disintegration apparatus.

• The limit for disintegration time varies depending on the type of tablet and its intended use.

• The USP sets standards for disintegration time for different types of tablets.

• Examples of disintegrants include croscarm...read more

BPH stands for Benign Prostatic Hyperplasia, a condition where the prostate gland enlarges and causes urinary problems.

• BPH is a common condition in older men

• Symptoms include frequent urination, difficulty starting and stopping urination, weak urine flow, and feeling like the bladder is not completely empty

• Treatment options include medication, minimally invasive procedures, and surgery

I am not familiar with DT machines, please provide more information.

• Please provide more information about the specific DT machine in question

• I can research and learn about the machine to better understand its capabilities and limitations

• Without more information, I cannot provide a specific answer

Natural products are compounds produced by living organisms, typically with therapeutic or medicinal properties.

• Natural products are compounds derived from plants, animals, or microorganisms.

• They are often used in traditional medicine and have therapeutic properties.

• Examples include aspirin from willow bark, penicillin from mold, and taxol from yew trees.