10+ Holidify Interview Questions and Answers

The interview questions cover topics such as self-introduction, developing methods for API to finished products, column chemistry, method validation, and ICH guidelines.

• For self-introduction, briefly talk about your background, education, and relevant experience.

• To develop a method for API to finished products, consider factors such as the desired assay and related substances, analytical techniques, and regulatory requirements.

• Column chemistry refers to the selection and opti...read more

Sink condition in dissolution refers to a state where the drug being dissolved is completely solubilized and no further dissolution occurs.

• Sink condition is achieved when the concentration of the drug in the dissolution medium is much lower than its solubility.

• It ensures that the dissolution process is not limited by the drug's solubility but by other factors like diffusion.

• Sink condition is important for accurate and reproducible dissolution testing of pharmaceutical product...read more

Possible reasons for not achieving mass balance in forced degradation

• Incomplete degradation of the analyte

• Formation of unknown degradation products

• Loss of analyte during sample preparation or analysis

• Inaccurate measurement of sample or standard volumes

• Errors in weighing or dilution of samples

• Contamination of samples or equipment

• Inadequate mixing or homogenization of samples

• Inefficient extraction of analyte from the matrix

• Inadequate stability of the analyte during the degradat...read more

pKa plays a crucial role in HPLC method development by determining the ionization state of analytes and their retention on the stationary phase.

• pKa helps in selecting the appropriate mobile phase pH for ionizable compounds.

• It influences the ionization of analytes, affecting their solubility and interaction with the stationary phase.

• pKa values aid in optimizing separation by adjusting the pH to enhance selectivity and resolution.

• For example, acidic compounds with low pKa value...read more

There are several types of GC capillary columns, including non-polar, polar, and specialty columns. The best type depends on the specific application and analytes being analyzed.

• Non-polar columns are best for separating non-polar compounds, such as hydrocarbons.

• Polar columns are ideal for separating polar compounds, such as alcohols and acids.

• Specialty columns, like chiral columns, are used for separating enantiomers.

• Factors to consider when choosing the best column include a...read more

Multimedia dissolution method can be developed by considering the physicochemical properties of the drug and the media used.

• Select appropriate media that mimic the physiological conditions of the drug's intended site of action

• Determine the solubility and stability of the drug in different media

• Optimize the pH and temperature of the media to enhance drug solubility

• Use appropriate equipment and techniques for sample preparation and analysis

• Validate the method according to regul...read more

pKa value determination and their role in development

• pKa value is a measure of the acidity or basicity of a compound

• It helps in understanding the chemical properties and behavior of a compound

• pKa values are important in drug development as they affect drug solubility, absorption, and stability

• Determining pKa values can aid in optimizing drug formulations and predicting drug-drug interactions

The pKa value of a product is calculated based on its acid dissociation constant and the concentration of the acid and its conjugate base.

• pKa is a measure of the acidity or basicity of a compound.

• It is calculated using the equation: pKa = -log10(Ka), where Ka is the acid dissociation constant.

• The acid dissociation constant is the ratio of the concentration of the dissociated form of the acid to the concentration of the undissociated form.

• pKa values can be determined experimen...read more

Developing an HPLC related substances method involves selecting appropriate column, mobile phase, and detection wavelength.

• Select a suitable column based on the analyte properties and separation requirements.

• Optimize the mobile phase composition and gradient program for efficient separation.

• Choose an appropriate detection wavelength based on the analyte's UV absorption properties.

• Validate the method by testing specificity, linearity, accuracy, precision, and robustness.

• Docume...read more

Bsc classification is a system used to categorize and classify living organisms based on their characteristics.

• Bsc classification stands for Biological Species Concept classification.

• It is used to group organisms based on their physical and genetic characteristics.

• The classification system includes Kingdom, Phylum, Class, Order, Family, Genus, and Species.

• Each level of classification is based on specific characteristics and traits.

• For example, humans are classified as Kingdom...read more