300+ Research Associate Interview Questions and Answers

Aldol condensation, Michael addition, Wittig reaction, Grignard reaction are some name reactions to form new c-c bond without metal catalyst.

• Aldol condensation involves the reaction of an enolate ion with an aldehyde or ketone to form a β-hydroxy carbonyl compound.

• Michael addition involves the addition of a nucleophile to an α,β-unsaturated carbonyl compound.

• Wittig reaction involves the reaction of a phosphorus ylide with an aldehyde or ketone to form an alkene.

• Grignard react...read more

To separate acid from an acid-base mixture in liquid state, we can use various methods such as solvent extraction, distillation, or ion exchange.

• Solvent extraction involves adding a solvent that selectively dissolves the acid, then separating the two layers.

• Distillation involves heating the mixture to vaporize the acid, then condensing it back into a separate container.

• Ion exchange involves passing the mixture through a resin that selectively binds to the acid, then eluting t...read more

I would investigate the cause and try alternative sterilization methods.

• Check if there is an issue with the equipment or the sterilization process

• Try alternative sterilization methods such as chemical sterilization or dry heat sterilization

• Consult with colleagues or experts in the field for advice

• Document the issue and any actions taken for future reference

NMR stands for Nuclear Magnetic Resonance. It is a spectroscopic technique used to study the magnetic properties of atomic nuclei.

• NMR works by applying a strong magnetic field to a sample and then measuring the absorption and emission of electromagnetic radiation by the nuclei in the sample.

• It is used in chemistry to determine the structure and composition of molecules, as well as to study chemical reactions.

• In medicine, NMR is used in magnetic resonance imaging (MRI) to prod...read more

I would choose to follow safety parameters over GLP.

• Safety of personnel and environment is of utmost importance

• GLP can be compromised temporarily but safety cannot

• Non-compliance with safety regulations can lead to serious consequences

Rest ethyl acetate in compound can be found by NMR using specific peaks and chemical shifts.

• Look for the peak at 1.0-1.2 ppm which corresponds to the ethyl group

• Look for the peak at 4.0-4.1 ppm which corresponds to the acetate group

• Subtract the integral of these peaks from the total integral to find the rest ethyl acetate

• Compare the chemical shifts of the peaks to known values for ethyl acetate

Answering questions related to upstream processing, including process flow, material and system use, FIT, CIP, SIP, bioreactor system, and role of raw material in media.

• Previous job responsibilities in upstream processing

• Process flow of upstream processing

• Make and MOC of the material and system use during operation

• FIT, CIP, and SIP

• Types of and part of bioreactor system

• Role of raw material in media

Column and mobile phase selection is based on the analyte properties and separation goals.

• Consider the analyte's physicochemical properties such as polarity, molecular weight, and stability.

• Choose a column with appropriate stationary phase chemistry (e.g., C18 for hydrophobic compounds).

• Optimize mobile phase composition (solvent ratio, pH, buffer) for desired separation.

• Consider the separation goals (resolution, selectivity, speed) and adjust column and mobile phase according...read more

The desired product of bromination on aniline is 2,4,6-tribromoaniline. The desired product of bromination on toluene is bromotoluene.

• Bromination on aniline results in the substitution of three bromine atoms on the aromatic ring, yielding 2,4,6-tribromoaniline.

• Bromination on toluene results in the substitution of one bromine atom on the aromatic ring, yielding bromotoluene.

Glycerol is used for long term preservation of bacterial cultures due to its ability to prevent ice crystal formation and cell damage.

• Glycerol acts as a cryoprotectant, preventing ice crystal formation and cell damage during freezing.

• It allows for long term storage of bacterial cultures at -80°C.

• Glycerol is added to the bacterial culture before freezing to a final concentration of 15-20%.

• It is a common practice in microbiology and biotechnology research.

• Other cryoprotectants ...read more

To prepare 0.1 N HCl and NaOH, you need to calculate the required amount of the respective chemicals and dilute them in water.

• Calculate the molar mass of HCl and NaOH

• Determine the volume of solution needed

• Use the formula: Normality (N) = (Number of equivalents / Volume of solution in liters)

• For 0.1 N HCl, calculate the number of equivalents of HCl and dilute it to the desired volume

• For NaOH, calculate the number of equivalents of NaOH and dilute it to the desired volume

• Always...read more

The choice of solvent can affect the UV spectrum by shifting the absorption peaks and changing the intensity.

• Solvents with different polarities can affect the UV spectrum differently.

• Solvents can cause shifts in the absorption peaks due to changes in the electronic environment.

• Solvents can also affect the intensity of the absorption peaks.

• The choice of solvent should be carefully considered to ensure accurate and consistent results.

• For example, water can cause shifts in the U...read more

The molecule can be converted into another molecule using reducing or oxidizing agents.

• Identify the functional groups present in the molecule

• Choose a suitable reducing or oxidizing agent based on the functional groups

• Perform the reaction under appropriate conditions

• Isolate and purify the product

• Examples: converting a ketone to an alcohol using NaBH4 as a reducing agent, converting an alcohol to a ketone using PCC as an oxidizing agent

Various company sources are used to give ESG risk ratings.

• Company reports and disclosures

• Third-party data providers

• News articles and media coverage

• Industry benchmarks and standards

• Stakeholder engagement and surveys

Pyrophoric chemicals can be handled and quenched by using inert gases, specialized containers, and proper disposal methods.

• Use inert gases such as nitrogen or argon to displace air and prevent ignition.

• Store and handle pyrophoric chemicals in specialized containers such as air-tight glass ampoules or sealed metal containers.

• Dispose of pyrophoric chemicals properly by contacting a hazardous waste disposal company.

• Examples of pyrophoric chemicals include alkali metals, metal hy...read more

pKa, LogP, and method development validation parameters are important in research associate work.

• pKa is a measure of acid strength and is important in drug design.

• LogP is a measure of lipophilicity and helps predict drug absorption and distribution.

• Method development validation parameters ensure accuracy and reliability of analytical methods.

• Examples of validation parameters include specificity, accuracy, precision, and linearity.

• These parameters are necessary to ensure the q...read more

Spectroscopy is the study of interaction between matter and electromagnetic radiation. UV spectroscopy measures absorption of UV light.

• Spectroscopy is used to identify and quantify substances based on their interaction with light.

• UV spectroscopy measures the absorption of UV light by molecules in a sample.

• UV spectroscopy is commonly used in the analysis of organic compounds.

• The range of UV spectroscopy is typically between 190-400 nm.

• UV spectroscopy can be used to determine t...read more

The Grignard reaction on an aldehyde will produce a secondary alcohol.

• The Grignard reaction involves the addition of a Grignard reagent (organomagnesium compound) to an aldehyde or ketone.

• The reaction proceeds through the formation of a carbon-carbon bond.

• In the case of an aldehyde, the product will be a secondary alcohol.

• For example, the reaction of formaldehyde (HCHO) with a Grignard reagent such as methylmagnesium bromide (CH3MgBr) will yield isopropyl alcohol (CH3CH(OH)CH...read more

H1NMR values are chemical shifts observed in nuclear magnetic resonance spectroscopy. NMR is a technique used to study the structure and properties of molecules.

• H1NMR values refer to the chemical shifts observed in proton nuclear magnetic resonance spectroscopy.

• NMR is a powerful analytical technique used to study the structure, dynamics, and properties of molecules.

• It involves the interaction of atomic nuclei with a strong magnetic field and radiofrequency radiation.

• Different...read more

Chromatography is a technique used to separate and analyze mixtures. HPLC is a type of chromatography used for high-resolution separation.

• Chromatography involves passing a mixture through a stationary phase, which separates the components based on their properties.

• HPLC uses high pressure to force the mixture through a column packed with a stationary phase, allowing for more precise separation.

• HPLC is commonly used in pharmaceuticals, forensics, and environmental testing to an...read more

Recombinant fermentation is a process of using microorganisms to produce recombinant proteins.

• Recombinant DNA technology is used to insert a gene into a microorganism's DNA

• The microorganism is then grown in a fermentation tank to produce the desired protein

• Examples include the production of insulin using E. coli bacteria

• Optimization of fermentation conditions is crucial for high yield and purity of the protein

Apart from Google, I would verify the background of a company by checking their financial statements, news articles, and industry reports.

• Check financial statements for revenue growth and profitability

• Read news articles for any controversies or legal issues

• Review industry reports for market trends and competition

• Check social media and online reviews for customer satisfaction

• Contact former employees or industry experts for insider information

Interpretation of NMR with coupling constant

• Coupling constant is the distance between two peaks in a multiplet

• It indicates the number of neighboring protons

• The coupling constant can be used to determine the stereochemistry of a molecule

• The coupling constant can also be used to determine the hybridization of a carbon atom

• The magnitude of the coupling constant depends on the strength of the spin-spin coupling

• A doublet has a coupling constant of around 7 Hz, a triplet around 7-9...read more

Sustainable finance refers to financial activities that promote sustainable economic growth while minimizing negative environmental and social impacts.

• Sustainable finance involves investing in companies that prioritize environmental and social responsibility.

• It also includes financing projects that promote sustainability, such as renewable energy and green infrastructure.

• Sustainable finance aims to balance economic growth with social and environmental responsibility.

• Examples ...read more

Pyrogen test is used to detect the presence of fever-causing substances in drugs or medical devices.

• Pyrogens are substances that can cause fever when introduced into the body.

• Rabbits are commonly used for pyrogen testing because they are sensitive to pyrogens.

• Other animals like guinea pigs and mice can also be used for pyrogen testing.

• Pyrogen testing is important to ensure the safety of drugs and medical devices before they are used on humans.

• The test involves injecting the s...read more

Method development of HPLC and Prep-HPLC involves optimizing parameters for separation and purification of compounds.

• Determine the appropriate stationary phase and mobile phase for the separation

• Optimize flow rate, column temperature, and gradient conditions

• Use appropriate detection methods such as UV or MS

• For Prep-HPLC, scale up the method and adjust parameters for larger column sizes

• Validate the method for accuracy, precision, and specificity

Reagents used in Suzuki coupling

• Palladium catalyst (e.g. Pd(PPh3)4)

• Organoboron compound (e.g. arylboronic acid)

• Base (e.g. potassium carbonate)

• Solvent (e.g. DMF, toluene)

I have experience handling various oxidation and reduction reagents in my previous research work.

• I have worked with common oxidizing agents such as potassium permanganate, hydrogen peroxide, and chromic acid.

• I have also handled reducing agents like sodium borohydride, lithium aluminum hydride, and diisobutylaluminum hydride.

• I am familiar with the safety precautions and proper handling techniques for these reagents.

• I have used these reagents in various chemical reactions to sy...read more

I am familiar with primary and secondary research, market research, and both quantitative and qualitative research methods.

• Primary research involves collecting data directly from the source, such as through surveys or interviews.

• Secondary research involves analyzing existing data, such as through literature reviews or data analysis.

• Market research involves studying consumer behavior and preferences to inform business decisions.

• Quantitative research involves collecting and ana...read more

Pulses are nutrient-rich seeds of legume plants, providing essential nutrients and health benefits.

• Pulses are a type of legume that includes beans, lentils, chickpeas, and peas.

• They are an excellent source of plant-based protein, fiber, vitamins, and minerals.

• Pulses are low in fat and cholesterol, making them a healthy choice for heart health.

• They are also rich in antioxidants, which help protect against chronic diseases.

• Pulses have a low glycemic index, making them suitable ...read more

The method for preparation of amide involves the reaction between a carboxylic acid and an amine.

• Amides can be prepared by the reaction of a carboxylic acid with an amine in the presence of a dehydrating agent.

• The reaction typically requires heating and can be catalyzed by an acid or base.

• The resulting product is an amide, which contains a carbonyl group bonded to a nitrogen atom.

• Example: The preparation of acetamide can be achieved by reacting acetic acid with ammonia in the...read more

I have handled various types of bacterial cultures.

• I have experience with both gram-positive and gram-negative bacteria.

• I have worked with aerobic and anaerobic bacteria.

• I have handled pathogenic bacteria such as E. coli and Salmonella.

• I have also worked with non-pathogenic bacteria such as Lactobacillus and Streptococcus.

• I have experience with bacterial cultures in both liquid and solid media.

Suzuki coupling byproduct is typically a biphenyl compound. LCMS shows peaks corresponding to reactants, products, and intermediates.

• Suzuki coupling byproduct is often a biphenyl compound formed due to side reactions.

• LCMS analysis of Suzuki reaction typically shows peaks corresponding to reactants, products, and intermediates.

• Monitoring the LCMS peaks can provide insights into the progress and efficiency of the Suzuki coupling reaction.

Pd coupling reactions involve the cross-coupling of two different carbon atoms using palladium as a catalyst.

• Pd coupling reactions are widely used in organic synthesis to form carbon-carbon bonds.

• Examples include Suzuki coupling, Sonogashira coupling, Heck reaction, and Stille coupling.

• These reactions typically involve a palladium catalyst, a base, and appropriate coupling partners.

• Suzuki coupling involves the reaction of an aryl halide with an organoboron compound.

• Sonogashir...read more

Changes made while transferring a product from same scale depend on various factors.

• The level of changes depends on the type of product being transferred.

• The level of changes also depends on the scale of transfer.

• Changes can be made in packaging, labeling, and formulation.

• Changes should be made while keeping in mind the regulatory requirements.

• Changes should be made while ensuring the quality and safety of the product.

Equipment sub class and classification vary according to unit operations.

• Equipment sub classes include mixing, separation, heat transfer, and reaction equipment.

• Mixing equipment includes agitators, blenders, and mixers.

• Separation equipment includes centrifuges, filters, and screens.

• Heat transfer equipment includes heat exchangers and boilers.

• Reaction equipment includes reactors and fermenters.

• Classification is based on the type of unit operation, such as distillation or cryst...read more

Canada is the top producer of lentils in the world, while India is the top producer of lentils in terms of domestic production.

• Canada is the largest exporter of lentils globally, accounting for about 35% of the total lentil exports.

• India is the largest producer of lentils in terms of domestic production, with a significant portion consumed within the country.

• Other major lentil-producing countries include Australia, Turkey, and the United States.

Exothermic reactions release heat energy while hydrolysis involves the breaking of chemical bonds using water.

• Exothermic reactions release heat energy to the surroundings, while hydrolysis involves the breaking of chemical bonds using water.

• Both exothermic reactions and hydrolysis are types of chemical reactions that involve energy changes.

• Exothermic reactions can sometimes involve hydrolysis, such as the hydrolysis of ATP to ADP which releases energy in cells.

Technical knowledge is essential for effective business intelligence. It helps in understanding data and making informed decisions.

• Technical knowledge enables the understanding of complex data sets and analysis

• It helps in identifying patterns and trends in data

• Technical knowledge aids in the selection and implementation of appropriate tools and technologies for data analysis

• It facilitates effective communication with technical teams and stakeholders

• For example, a research ass...read more

Yes, I have experience in biofertilizers production.

• I have worked on a research project where I produced biofertilizers using organic waste materials.

• I have knowledge of the different techniques and processes involved in biofertilizers production.

• I have successfully conducted experiments to optimize the production of biofertilizers.

• I am familiar with the quality control measures required for biofertilizers production.

• I have collaborated with a team of scientists and researche...read more

Precautions taken during analysis

• Ensuring accurate and reliable data collection

• Maintaining proper documentation and record-keeping

• Following established protocols and procedures

• Implementing quality control measures

• Validating and verifying results

• Protecting confidentiality and privacy of data

• Considering potential biases and limitations

• Applying statistical analysis techniques appropriately

Disintegration is the breakdown of tablet into smaller particles while dissolution is the process of drug release from the tablet.

• Disintegration is a physical process while dissolution is a chemical process.

• Disintegration time is important for immediate release tablets while dissolution test is important for extended release tablets.

• Dissolution test helps to determine the rate and extent of drug release from the tablet.

• Dissolution test is important for quality control and to ...read more

Synthesized 2-methylcyclohexanol from cyclohexene using acid-catalyzed hydration.

• Started with cyclohexene and added concentrated sulfuric acid to protonate the double bond.

• Water was then added to the reaction mixture, which attacked the carbocation intermediate to form a tertiary alcohol.

• The product was then extracted and purified via distillation.

• 2-methylcyclohexanol was characterized using IR and NMR spectroscopy.

The biaryl product can be synthesized through a variety of methods.

• One common method is the Suzuki-Miyaura cross-coupling reaction.

• Another method is the Buchwald-Hartwig amination reaction.

• Transition metal-catalyzed C-H activation can also be used.

• Examples of biaryl products include biphenyl and terphenyl.

The basic analysis for choosing a power generation unit involves evaluating factors such as cost, efficiency, environmental impact, and availability of resources.

• Consider the cost of building and operating the power generation unit.

• Evaluate the efficiency of converting the chosen material (e.g. coal) into electricity.

• Assess the environmental impact of the power generation unit, including emissions and waste disposal.

• Take into account the availability and sustainability of the...read more

Nutrition refers to the process of providing or obtaining the food necessary for health and growth. Plants require 17 essential nutrients for proper growth and development.

• Nutrition is the process of providing or obtaining the food necessary for health and growth

• Plants require 17 essential nutrients for proper growth and development

• These nutrients include macronutrients like nitrogen, phosphorus, and potassium, as well as micronutrients like iron, zinc, and manganese

Microsoft Excel is a software product used for creating spreadsheets and analyzing data.

• Allows users to organize data into rows and columns

• Provides tools for performing calculations and creating charts

• Commonly used in business for financial analysis and reporting