Glenmark Life Sciences Research Scientist Interview Questions and Answers for Experienced

The percentage of buffer used in mobile phase and the use of SLS in disso and its allowed percentage.

• The percentage of buffer used in the mobile phase depends on the specific experiment and the desired separation of compounds.

• Buffer is used in the mobile phase to maintain the pH and ionic strength, which can affect the separation and stability of analytes.

• The percentage of buffer can range from 5% to 50% or more, depen...read more

The sink condition is decided based on various factors such as the purpose of the sink, the type of research being conducted, and the desired outcome.

• Consider the purpose of the sink - Is it for disposal, storage, or analysis of substances?

• Evaluate the type of research being conducted - Does it involve chemical reactions, biological samples, or physical measurements?

• Assess the desired outcome - Are you looking for pres...read more

The criteria/limit for impurity recovery depends on the specific research study and the desired purity level.

• The criteria for impurity recovery may include the maximum allowable impurity concentration or percentage.

• The limit for impurity recovery can be determined based on regulatory guidelines or industry standards.

• The desired purity level may vary depending on the application or intended use of the research findings.

• ...read more

Mass balance is a principle used in various scientific disciplines to account for the mass of substances in a system.

• Mass balance is based on the principle of conservation of mass, which states that mass cannot be created or destroyed, only transferred or transformed.

• It involves tracking the inputs, outputs, and accumulation of mass within a system.

• Mass balance equations are commonly used in environmental science, chem...read more

RF values are important in chromatography to identify and separate compounds.

• RF value is the ratio of the distance traveled by a compound to the distance traveled by the solvent front in chromatography.

• It helps in identifying and separating compounds based on their polarity and solubility.

• RF values can be used to compare compounds and determine their purity.

• For example, in thin-layer chromatography, RF values are used ...read more

Preparing buffers, differences between NP-HPLC and RP-HPLC, and uses of potentiometric titrations.

• Buffers are prepared by mixing a weak acid and its conjugate base or a weak base and its conjugate acid.

• NP-HPLC separates non-polar compounds while RP-HPLC separates polar compounds.

• Potentiometric titrations are used to determine the concentration of an unknown solution by measuring the potential difference between two ele...read more

Qbd approach involves designing and developing a formulation and process based on quality attributes and risk assessment.

• Qbd stands for Quality by Design

• It involves identifying critical quality attributes (CQAs) and critical process parameters (CPPs)

• Risk assessment is done to identify potential sources of variability and their impact on CQAs

• Formulation and process are then designed and developed to ensure consistent qu...read more