BioNome Intern Interview Questions and Answers

Protein structure refers to the three-dimensional arrangement of atoms in a protein molecule, which determines its function.

• Proteins are made up of amino acids, which are linked together by peptide bonds.

• The primary structure of a protein is the linear sequence of amino acids.

• The secondary structure refers to local folding patterns, such as alpha helices and beta sheets.

• The tertiary structure is the overall three-dimen...read more

Ion exchange chromatography separates proteins based on their net charge, with different types and exchange capacities. pH affects protein binding.

• Ion exchange chromatography separates proteins based on their net charge

• Types include cation exchange (positively charged proteins bind to negatively charged resin) and anion exchange (negatively charged proteins bind to positively charged resin)

• Exchange capacity refers to t...read more

Protein structure refers to the three-dimensional arrangement of atoms in a protein molecule, which determines its function.

• Proteins are made up of amino acids, which are linked together by peptide bonds.

• The primary structure of a protein is the linear sequence of amino acids.

• The secondary structure refers to local folding patterns, such as alpha helices and beta sheets.

• The tertiary structure is the overall three-dimen...read more

Passaging cells involves detaching cells from a culture vessel, diluting them, and transferring them to a new vessel to allow for continued growth.

• Use a sterile technique to detach cells from the culture vessel

• Collect the cells in a centrifuge tube and centrifuge to pellet the cells

• Resuspend the cells in fresh media and calculate the appropriate dilution factor

• Transfer the diluted cells to a new culture vessel and incu