Gujarat Biotechnology Research Centre (GBRC) Interview Questions, Process, and Tips

100 objective questions to answer in 1 hour

Q1. Role of DEPC in RNA isolation
Ans. 
DEPC is used in RNA isolation to inactivate RNases and prevent RNA degradation.
• DEPC (diethyl pyrocarbonate) is a chemical used to treat water and equipment to inactivate RNases, which can degrade RNA during isolation.

• DEPC-treated water is used to make solutions for RNA isolation procedures to ensure RNase-free conditions.

• DEPC can be used to treat glassware, pipettes, and other equipment to prevent RNase contamination d...read more
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Q2. What is RIL population
Ans. 
RIL population refers to Recombinant Inbred Line population, which is a set of inbred lines derived from crossing two genetically different parents and then self-pollinating the offspring for several generations.
• RIL population is commonly used in genetics research to study genetic variation and map genes of interest.

• Each line in the RIL population is genetically unique and can be used to identify genetic loci associate...read more
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Written exam was held followed by interview

Q1. What is pcr in biotechnology
Ans. 
PCR stands for polymerase chain reaction, a technique used in biotechnology to amplify a specific DNA sequence.
• PCR is a method used to make multiple copies of a specific DNA segment.

• It involves cycles of heating and cooling to denature, anneal, and extend DNA strands.

• PCR is commonly used in research, forensics, medical diagnostics, and genetic testing.

• Examples of PCR applications include identifying genetic disorders, ...read more
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Q2. What is the genetic engineering
Ans. 
Genetic engineering is the process of manipulating an organism's DNA to create desired traits or outcomes.
• Involves altering the genetic material of an organism

• Can be used to create genetically modified organisms (GMOs)

• Used in various fields such as agriculture, medicine, and research

• Examples include creating crops with increased resistance to pests or diseases
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Questions were asked from all backgrounds

Q1. Personal review and experience
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Q2. Past experience and knowledge
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Q1. Fermentation technology
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Q2. Saccharomyces cerevisiae
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Q1. Response surface methodology
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Q2. Optimization and product development
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Q1. LCMS and GCMS and CID
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Q2. Application in Detection of metronidazole, aflatoxin, albendazole, fenbendazole
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Q1. PCR realated
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