Alkem Laboratories Quality Analyst Interview Questions, Process, and Tips

System suitability for HPLC refers to the ability of the system to provide accurate and precise results consistently.

• System suitability tests are performed to ensure that the HPLC system is working properly.

• Parameters such as resolution, peak symmetry, and retention time are evaluated.

• Acceptance criteria are established based on the method being used.

• Examples of system suitability tests include replicate injections, st...read more

Troubleshooting for HPLC

• Check for leaks in the system

• Verify the column is properly installed and conditioned

• Ensure the mobile phase is properly prepared and filtered

• Check for air bubbles in the system

• Verify the detector is properly calibrated

• Inspect the sample preparation process

• Check for any software or hardware malfunctions

UV spectrophotometer principle and calibration

• UV spectrophotometer measures the absorbance or transmittance of light in the UV range

• Principle: It uses a UV light source to pass light through a sample and measures the intensity of light before and after passing through the sample

• Calibration: Involves setting the instrument to zero absorbance with a blank solution and verifying accuracy with known standard solutions

• Calib...read more

pH stands for potential of hydrogen.

• pH is a measure of the acidity or alkalinity of a solution.

• It is a logarithmic scale ranging from 0 to 14.

• A pH of 7 is considered neutral, below 7 is acidic, and above 7 is alkaline.

• pH is commonly used in various fields such as chemistry, biology, and environmental science.

The aufbau principle is a rule in quantum mechanics that states how electrons fill atomic orbitals.

• The principle states that electrons fill the lowest energy levels first before moving to higher energy levels.

• It follows the order of filling orbitals: 1s, 2s, 2p, 3s, 3p, 4s, etc.

• Each orbital can hold a maximum of two electrons with opposite spins.

• For example, the electron configuration of carbon (atomic number 6) is 1s²

Electron pairing occurs after half-filled orbitals in a subshell.

• Electron pairing is a phenomenon where two electrons occupy the same orbital.

• This occurs after each orbital in a subshell has been half-filled with electrons.

• The pairing of electrons is due to the principle of maximum multiplicity.

• For example, in the p subshell, electron pairing occurs after the first three orbitals have been half-filled with electrons.