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Alkem Laboratories Quality Analyst Interview Questions and Answers

Updated 27 May 2024

Q1. The pairing of electron started after half filled of each orbital in a subshell.

Ans.

Electron pairing occurs after half-filled orbitals in a subshell.

  • Electron pairing is a phenomenon where two electrons occupy the same orbital.

  • This occurs after each orbital in a subshell has been half-filled with electrons.

  • The pairing of electrons is due to the principle of maximum multiplicity.

  • For example, in the p subshell, electron pairing occurs after the first three orbitals have been half-filled with electrons.

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Q2. Principle and calibration of uv spectrophotometer

Ans.

UV spectrophotometer principle and calibration

  • UV spectrophotometer measures the absorbance or transmittance of light in the UV range

  • Principle: It uses a UV light source to pass light through a sample and measures the intensity of light before and after passing through the sample

  • Calibration: Involves setting the instrument to zero absorbance with a blank solution and verifying accuracy with known standard solutions

  • Calibration ensures accurate measurement and reliable results

  • Ex...read more

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Q3. Acceptance value calculation for Content uniformity

Ans.

Acceptance value calculation for Content uniformity is a critical aspect of quality analysis in pharmaceutical industry.

  • Acceptance value is calculated using the formula: Acceptance value = |100 - (100 * (X̄/μ))| + k * σ, where X̄ is the sample mean, μ is the target value, k is the acceptance criteria, and σ is the standard deviation.

  • Acceptance criteria for content uniformity is typically set at 15%.

  • The acceptance value should be within the acceptance criteria for the content to...read more

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Q4. What is ph stands for

Ans.

pH stands for potential of hydrogen.

  • pH is a measure of the acidity or alkalinity of a solution.

  • It is a logarithmic scale ranging from 0 to 14.

  • A pH of 7 is considered neutral, below 7 is acidic, and above 7 is alkaline.

  • pH is commonly used in various fields such as chemistry, biology, and environmental science.

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Q5. What is aufbau principle

Ans.

The aufbau principle is a rule in quantum mechanics that states how electrons fill atomic orbitals.

  • The principle states that electrons fill the lowest energy levels first before moving to higher energy levels.

  • It follows the order of filling orbitals: 1s, 2s, 2p, 3s, 3p, 4s, etc.

  • Each orbital can hold a maximum of two electrons with opposite spins.

  • For example, the electron configuration of carbon (atomic number 6) is 1s² 2s² 2p².

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Q6. System suitability for hplc

Ans.

System suitability for HPLC refers to the ability of the system to provide accurate and precise results consistently.

  • System suitability tests are performed to ensure that the HPLC system is working properly.

  • Parameters such as resolution, peak symmetry, and retention time are evaluated.

  • Acceptance criteria are established based on the method being used.

  • Examples of system suitability tests include replicate injections, standard solution analysis, and column performance tests.

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Q7. Troubleshooting for hplc

Ans.

Troubleshooting for HPLC

  • Check for leaks in the system

  • Verify the column is properly installed and conditioned

  • Ensure the mobile phase is properly prepared and filtered

  • Check for air bubbles in the system

  • Verify the detector is properly calibrated

  • Inspect the sample preparation process

  • Check for any software or hardware malfunctions

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