UV spectrophotometer principle and calibration

• UV spectrophotometer measures the absorbance or transmittance of light in the UV range

• Principle: It uses a UV light source to pass light through a sample ...read more

Principle of UV-spectrophotometer:

"When a beam of monochromatic light is passing through a homogeneous absorbing medium the rate of decrease in intensity of light is directly proportional to the concentration and path length of the absorbing medium."

For UV calibration as per USP is done on five parameters.

1. Control of Wavelength

2. Control of Absorbance

3. Limit of Stray light

4. Resolution power

5. Baseline Flatness

Its lengthy answer can check this here Calibration of UV-spectrophotometer