Zeiss Pharma QC Chemist Interview Questions and Answers

Total 30 Questions (15 Aptitude and 15 Technical Questions)

Q1. Any Nameing reaction Mechanism
Ans. 
The Grignard reaction involves the addition of an organomagnesium halide to a carbonyl group.
• The reaction is named after French chemist Victor Grignard.

• It is used to form carbon-carbon bonds and is a key reaction in organic synthesis.

• An example is the synthesis of triphenylmethanol from benzophenone and phenylmagnesium bromide.

• The reaction proceeds through the formation of a Grignard reagent, which then attacks the car...read more
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Q2. What is Titration, What is pH
Ans. 
Titration is a technique to determine the concentration of a substance in a solution. pH is a measure of acidity or basicity of a solution.
• Titration involves adding a solution of known concentration to a solution of unknown concentration until the reaction is complete.

• pH is measured on a scale of 0-14, with 7 being neutral, below 7 acidic, and above 7 basic.

• Titration can be used to determine the concentration of acids,...read more
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Q1. Waiting For 2 Months
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HPLc,GC, IR,UV,And basic chemistry related questions

Q1. Chemical Name and formula
Ans. 
Chemical name and formula are identifiers for specific chemical compounds.
• Chemical name is the common name given to a chemical compound.

• Chemical formula represents the elements and their ratios in a compound.

• Example: Water - Chemical name, H2O - Chemical formula.
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Q2. How to make 0.1M HCL
Ans. 
To make 0.1M HCL, dilute concentrated hydrochloric acid with water.
• Dilute 1 part of concentrated HCL with 9 parts of water to make 0.1M HCL solution.

• Always add acid to water slowly to prevent splashing and heat generation.

• Use proper protective equipment such as gloves and goggles when handling acids.
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Q1. Trouble shooting of HPLC
Ans. 
Troubleshooting of HPLC involves identifying and resolving issues with the high-performance liquid chromatography system.
• Check for leaks in the system and tighten connections if necessary.

• Inspect and clean the columns regularly to ensure optimal performance.

• Calibrate the detector and pump to maintain accuracy of results.

• Troubleshoot error messages on the HPLC software by checking system settings and parameters.

• Consult ...read more
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Q2. Why use caffeine for calibration of HPLC
Ans. 
Caffeine is used for calibration of HPLC due to its well-defined retention time and peak shape.
• Caffeine has a well-defined retention time, making it easy to identify and calibrate the HPLC system.

• Caffeine produces a sharp and symmetrical peak, which helps in accurately determining the system's performance.

• It is a commonly used compound for calibration in HPLC due to its stability and reproducibility.

• Using caffeine as a...read more
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Q1. What is pH meter , How to calibrate pH meter
Ans. 
A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.
• pH meter measures the concentration of hydrogen ions in a solution

• Calibration involves adjusting the pH meter to known standard solutions

• Regular calibration ensures accurate readings over time
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Q2. Explain the parts of HPLC
Ans. 
HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.
• HPLC involves a pump to push the liquid solvent through the system.

• It includes an injector to introduce the sample into the mobile phase.

• The column separates the components of the sample based on their interactions with the stationary phase.

• A detector measures the amount of analyte in the eluent.

• ...read more
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Q1. What are the buffer used in HPLC
Ans. 
Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.
• Phosphate buffers are commonly used in HPLC for separating acidic compounds.

• Acetate buffers are used for separating basic compounds.

• Citrate buffers are used for separating metal ions.

• Other common buffers used in HPLC include ammonium acetate and ammonium formate.
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Q2. How to calibrate HPLC
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HPLC calibration involves adjusting parameters to ensure accurate and reliable results.
• Prepare standard solutions of known concentrations

• Run the standard solutions through the HPLC system

• Compare the results to expected values and adjust parameters as needed

• Repeat the process until accurate and consistent results are obtained
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