Unichem Laboratories Quality Control Officer Trainee Interview Questions and Answers

TLC stands for Thin Layer Chromatography, a technique used to separate and analyze mixtures of chemicals.

• TLC involves a stationary phase (thin layer of adsorbent material) and a mobile phase (solvent).

• The sample mixture is spotted on the TLC plate and the plate is placed in a developing chamber with the mobile phase.

• Different components of the mixture move at different rates on the plate, allowing for separation and id...read more

Disintegration is the breakdown of a solid dosage form into smaller particles, while dissolution is the process by which a solid substance dissolves in a liquid.

• Disintegration is a physical process, while dissolution is a chemical process.

• Disintegration involves the breaking down of a tablet or capsule into smaller pieces, while dissolution involves the solubilization of the active ingredient.

• Disintegration testing is ...read more

Detectors in HPLC include UV-Vis, fluorescence, refractive index, and mass spectrometry.

• UV-Vis detector measures absorbance of UV or visible light by the analyte.

• Fluorescence detector measures fluorescence emitted by the analyte when excited by light.

• Refractive index detector measures changes in refractive index of the eluent.

• Mass spectrometry detector measures mass-to-charge ratio of ions produced from analyte.

UV and FTIR principles are analytical techniques used for identifying and analyzing chemical compounds based on their absorption of ultraviolet-visible light and infrared radiation, respectively.

• UV spectroscopy involves measuring the absorption of UV or visible light by a sample to determine its concentration or purity.

• FTIR spectroscopy measures the absorption of infrared radiation by a sample to identify functional gr...read more

Quality control is a process used to ensure that products or services meet specific standards and requirements.

• Quality control involves monitoring and testing products or services to identify defects or errors.

• It aims to prevent defects from reaching customers and ensure consistent quality.

• Examples of quality control methods include statistical process control, inspection, and testing.

• Quality control is essential in in...read more

Handle the instrument with care and follow proper procedures

• Always read the instruction manual before using the instrument

• Ensure the instrument is calibrated and maintained regularly

• Use the instrument only for its intended purpose

• Clean the instrument after each use to prevent contamination

• Store the instrument in a safe and secure location when not in use

Gas chromatography is a technique used to separate and analyze compounds in a gas mixture.

• Gas chromatography involves a sample being vaporized and injected into a column with a stationary phase.

• Compounds in the sample interact differently with the stationary phase, causing them to separate based on their properties.

• The separated compounds are detected as they exit the column, allowing for identification and quantificat...read more

There are typically three main departments in an HPLC machine: the pump, the injector, and the detector.

• Pump department is responsible for delivering the mobile phase at a constant flow rate.

• Injector department is where the sample is introduced into the mobile phase stream.

• Detector department is used to detect the separated components of the sample as they elute from the column.

• Some HPLC machines may also have addition...read more