TÜV SÜD Analytical Qc Chemist Interview Questions, Process, and Tips

HPLC and GC differ in their mobile phase, stationary phase, and sample types. GC uses a gas phase while HPLC uses a liquid phase.

• HPLC uses a liquid mobile phase while GC uses a gas mobile phase

• GC uses a packed column while HPLC uses a solid or porous stationary phase

• GC is used for volatile and semi-volatile compounds while HPLC is used for non-volatile compounds

• Chapters studied in MSC depend on the program and universi...read more

To prepare a 1M solution, dissolve the molecular weight of the solute in 1 liter of solvent.

• Determine the molecular weight of the solute

• Measure 1 liter of solvent

• Dissolve the calculated amount of solute in the solvent

• Mix thoroughly to ensure homogeneity

Both HPLC and GC have their own advantages and limitations, and the choice depends on the specific analytical requirements.

• HPLC is better for analyzing polar and non-volatile compounds, while GC is better for analyzing volatile compounds.

• HPLC is more sensitive and can detect lower concentrations of analytes, while GC has a faster analysis time.

• The choice between HPLC and GC also depends on the sample matrix and the req...read more

UV stands for ultraviolet and the principle of UV is the absorption of light in the UV region by molecules.

• UV spectroscopy is used to determine the concentration of a substance in a sample by measuring the amount of UV light absorbed by the sample.

• The principle of UV is based on the fact that molecules absorb light in the UV region of the electromagnetic spectrum.

• UV spectroscopy is commonly used in analytical chemistry

Detectors used in HPLC, GC and UV include UV-Vis, fluorescence, refractive index, mass spectrometry, and flame ionization.

• UV-Vis detectors measure the absorbance of light by the sample at a specific wavelength.

• Fluorescence detectors measure the fluorescence emitted by the sample when excited by light.

• Refractive index detectors measure the change in refractive index of the mobile phase caused by the sample.

• Mass spectrom...read more