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HPLC is a technique used to separate, identify, and quantify components in a mixture based on their interactions with a stationary phase and a mobile phase.
HPLC stands for High-Performance Liquid Chromatography.
It involves the use of a stationary phase (usually a column packed with particles) and a mobile phase (liquid solvent).
The sample mixture is injected into the HPLC system and carried by the mobile phase through ...
Troubleshooting of HPLC involves identifying and resolving issues with the high-performance liquid chromatography system.
Check for leaks in the system and ensure proper connections
Inspect and clean the column to remove any blockages or contaminants
Verify the mobile phase composition and ensure it is prepared correctly
Calibrate and check the detector for proper functioning
Troubleshoot any issues with the pump, such as a...
Calibration of Balance pH KF
Calibration of a balance involves ensuring its accuracy and precision
pH calibration involves adjusting the pH meter to accurately measure pH levels
KF calibration refers to calibrating a Karl Fischer titrator to accurately measure water content
Each calibration requires specific standards and procedures
Regular calibration is necessary to maintain accurate measurements
I applied via Naukri.com and was interviewed in Jan 2022. There was 1 interview round.
I applied via Company Website and was interviewed before May 2021. There were 4 interview rounds.
I applied via Referral and was interviewed in Apr 2021. There were 4 interview rounds.
Tips can range from a few dollars to a percentage of the total bill depending on the service provided.
Tips are usually given in the service industry such as restaurants, salons, and hotels.
In restaurants, tips can range from 15% to 20% of the total bill.
In salons, tips can range from $5 to $20 depending on the service provided.
In hotels, tips can range from $1 to $5 per day for housekeeping and $2 to $10 for room servi...
Syngene International interview questions for popular designations
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Get interview-ready with Top Syngene International Interview Questions
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I applied via Company Website and was interviewed in Oct 2021. There were 3 interview rounds.
I applied via Walk-in and was interviewed before Dec 2021. There were 3 interview rounds.
Questions on attitude, interest and team work etc were asked
I applied via Company Website and was interviewed in May 2021. There were 3 interview rounds.
Selective reduction of acid in presence of ester
Use a reducing agent that selectively reduces acid over ester
Common reducing agents are LiAlH4 and NaBH4
LiAlH4 reduces both acid and ester, but in presence of acid it selectively reduces acid
NaBH4 reduces only acid, not ester
Example: Reduction of benzoic acid to benzyl alcohol in presence of benzyl ester using LiAlH4
I applied via Referral and was interviewed in May 2021. There were 3 interview rounds.
Interpretation of NMR with coupling constant
Coupling constant is the distance between two peaks in a multiplet
It indicates the number of neighboring protons
The coupling constant can be used to determine the stereochemistry of a molecule
The coupling constant can also be used to determine the hybridization of a carbon atom
The magnitude of the coupling constant depends on the strength of the spin-spin coupling
A doublet ha...
The molecule can be converted into another molecule using reducing or oxidizing agents.
Identify the functional groups present in the molecule
Choose a suitable reducing or oxidizing agent based on the functional groups
Perform the reaction under appropriate conditions
Isolate and purify the product
Examples: converting a ketone to an alcohol using NaBH4 as a reducing agent, converting an alcohol to a ketone using PCC as an
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