SRL Diagnostics Consultant Pathologist Interview Questions and Answers

Peak purity is the measure of the homogeneity of a peak in a chromatogram, while % area normalization is a method to compare the relative amounts of different peaks in a chromatogram.

• Peak purity is determined by comparing the spectra at different points within a peak to ensure they are similar, indicating a pure compound.

• % area normalization involves adjusting the peak areas in a chromatogram to a common reference poin...read more

PDA detector measures absorbance at multiple wavelengths, while DAD detector measures absorbance at all wavelengths simultaneously.

• PDA detector uses a photodiode array to measure absorbance at multiple wavelengths, allowing for more detailed analysis.

• DAD detector uses a diode array to measure absorbance at all wavelengths simultaneously, providing faster and more comprehensive data.

• PDA detector is more suitable for app...read more

Beer's Ambert Law states that the absorbance of a substance is directly proportional to its concentration and the path length of the light through the sample.

• Beer's Ambert Law is commonly used in spectrophotometry to determine the concentration of a substance in a solution.

• The law is expressed as A = εlc, where A is the absorbance, ε is the molar absorptivity, l is the path length, and c is the concentration.

• It is appl...read more

Developing RS method development involves understanding the research objectives, selecting appropriate techniques, optimizing parameters, and validating the method.

• Understand the research objectives and requirements

• Select appropriate techniques based on the sample and analyte properties

• Optimize method parameters such as mobile phase composition, column temperature, and flow rate

• Validate the method by assessing paramete...read more