100+ Medical Laboratory Technician Interview Questions and Answers

Yes, I have experience in fully automated instrument handling, quality control/calibration, and audit handling.

• I have experience in operating fully automated instruments for bio and immuno assays.

• I am proficient in performing quality control and calibration procedures to ensure accurate results.

• I have experience in preparing and maintaining documentation for laboratory procedures.

• I am familiar with audit handling and NABL requirements.

• For example, in my previous job, I was re...read more

Perform a complete blood count (CBC) to check for abnormalities in other blood components.

• Perform a CBC to check for abnormalities in other blood components such as red blood cells, white blood cells, and hemoglobin.

• Other tests that may be done include a blood smear to examine the shape and size of blood cells, and a coagulation test to check for clotting disorders.

• Platelet count may also be repeated to confirm the initial result and rule out any errors or fluctuations.

• Exampl...read more

Order of draw is the sequence of tubes to be drawn during venipuncture to avoid cross-contamination and inaccurate test results.

• The order of draw is: cultural, citrate, plain, heparin, EDTA, fluoride

• Cultural tube is used for blood cultures to detect bacterial infections

• Citrate tube is used for coagulation studies

• Plain tube is used for serum tests

• Heparin tube is used for plasma tests

• EDTA tube is used for hematology tests

• Fluoride tube is used for glucose tests

• Following the orde...read more

Hyperglycemia symptoms include increased thirst, frequent urination, blurred vision, fatigue, and headaches.

• Increased thirst

• Frequent urination

• Blurred vision

• Fatigue

• Headaches

High lymphocyte count

• Lymphocytes are a type of white blood cell

• High lymphocyte count can indicate infection, autoimmune disease, or cancer

• Further testing may be needed to determine the cause

Electrolytes include sodium, potassium, chloride, bicarbonate, calcium, and magnesium. Normal range of WBC is 4,500-11,000 cells/mcL.

• Electrolytes are ions that carry an electrical charge and are essential for various bodily functions.

• Sodium and potassium are important for nerve and muscle function.

• Chloride helps maintain fluid balance and acid-base balance.

• Bicarbonate helps regulate pH levels in the body.

• Calcium and magnesium are important for bone health and muscle function....read more

The anticoagulant in liquid form is heparin.

• Heparin is a commonly used anticoagulant in medical laboratories.

• It is used to prevent blood clots and is available in liquid form.

• Heparin is often used in blood tests to prevent clotting of the sample.

• Other anticoagulants like EDTA and citrate are available in liquid form as well.

Lipid profile includes tests for cholesterol, triglycerides, HDL, LDL, and VLDL.

• Cholesterol test measures total cholesterol in the blood.

• Triglycerides test measures the amount of fat in the blood.

• HDL test measures the good cholesterol in the blood.

• LDL test measures the bad cholesterol in the blood.

• VLDL test measures the very low-density lipoprotein in the blood.

• Lipid profile is used to assess the risk of heart disease and stroke.

CSF lactic acid is important for diagnosing bacterial meningitis and other central nervous system infections.

• CSF lactic acid levels can help differentiate between bacterial and viral meningitis.

• Elevated CSF lactic acid levels indicate bacterial meningitis or other CNS infections.

• Low CSF lactic acid levels are typically seen in viral meningitis or non-infectious conditions.

• CSF lactic acid measurement is a valuable tool in guiding appropriate treatment decisions.

• Example: A pati...read more

Normal range of haemoglobin and best method for estimation

• Normal range of haemoglobin in adult males is 13.5-17.5 g/dL and in adult females is 12.0-15.5 g/dL

• The best method for estimation of haemoglobin is the cyanmethemoglobin method

• Other methods include the Sahli's method, Hemiglobincyanide method, and Hemoglobin electrophoresis

Maintaining quality in lab is crucial for accurate results and patient safety.

• Regular calibration and maintenance of equipment

• Strict adherence to standard operating procedures

• Proper training and supervision of lab personnel

• Regular quality control checks and participation in proficiency testing programs

• Maintaining a clean and organized work environment

• Documenting all procedures and results accurately

• Continuous improvement through ongoing education and staying up-to-date with i...read more

ESR stands for erythrocyte sedimentation rate. It is a blood test that measures the rate at which red blood cells settle in a test tube.

• ESR is a non-specific test that helps to detect inflammation in the body.

• It is used to diagnose and monitor the progress of conditions such as infections, autoimmune diseases, and cancer.

• The test involves taking a sample of blood and measuring the distance that the red blood cells settle in a tube over a period of time.

• A higher ESR value indi...read more

PPE stands for Personal Protective Equipment.

• PPE refers to specialized clothing or equipment worn by individuals to protect themselves from potential hazards in the workplace.

• It includes items such as gloves, masks, goggles, face shields, gowns, and respirators.

• PPE is essential in medical laboratories to prevent exposure to infectious materials, chemicals, or other dangers.

• Examples of PPE in a medical laboratory setting include wearing gloves when handling specimens, using a ...read more

Anticoagulant is a substance that prevents blood from clotting. Natural anticoagulant example is heparin.

• Anticoagulant is a substance that inhibits the clotting of blood.

• It is commonly used in medical procedures to prevent blood clots.

• Heparin is a natural anticoagulant found in the body.

• Other examples of natural anticoagulants include antithrombin and protein C.

Fixation is necessary for slide preparation for morphology as it preserves the cellular structure and prevents degradation.

• Fixation helps to preserve the cellular structure of the sample.

• It prevents degradation of the sample during slide preparation.

• It helps to maintain the morphology of the cells for accurate analysis.

• Common fixatives include formalin, ethanol, and methanol.

• Different fixatives may be used for different types of samples, such as tissues or cells.

• Fixation is a...read more

ESR stands for erythrocyte sedimentation rate.

• ESR is a blood test that measures how quickly red blood cells settle at the bottom of a test tube.

• It is a non-specific test used to detect inflammation in the body.

• ESR is often used to monitor conditions such as rheumatoid arthritis, lupus, and infections.

• The normal range for ESR varies depending on age and gender.

• High ESR levels can indicate the presence of an underlying medical condition.

The WHO recommended haemoglobin estimation method is the cyanmethemoglobin method.

• The cyanmethemoglobin method involves converting hemoglobin to cyanmethemoglobin, which is then measured spectrophotometrically.

• This method is considered to be the gold standard for hemoglobin estimation.

• It provides accurate and reliable results.

• Other methods like the Sahli's method or the Hemocue method may also be used, but the cyanmethemoglobin method is recommended by the WHO.

Acidosis is a condition where the blood pH is too low and alkalosis is a condition where the blood pH is too high.

• Acidosis occurs when there is an excess of acid in the blood or a loss of bicarbonate.

• Alkalosis occurs when there is an excess of base in the blood or a loss of acid.

• Examples of acidosis include diabetic ketoacidosis and lactic acidosis.

• Examples of alkalosis include hyperventilation and vomiting.

• Both acidosis and alkalosis can have serious health consequences if l...read more

The percentage of agar used for solid media varies depending on the type of media and the purpose of the culture.

• Agar concentration typically ranges from 1-2% for most bacterial cultures.

• Some specialized media may require higher agar concentrations, up to 5%.

• Agar concentration can also affect the hardness of the media, with higher concentrations resulting in firmer gels.

• The percentage of agar can be adjusted based on the specific needs of the culture, such as promoting the gr...read more

Tests for fungal infection in patients

• Collect samples from the affected area

• Perform a fungal culture test

• Conduct a microscopic examination of the sample

• Perform a blood test to detect fungal antibodies

• Use PCR (polymerase chain reaction) to detect fungal DNA

• Perform a skin test to check for an allergic reaction to fungal antigens

I manage lab quality by implementing quality control measures, ensuring accuracy of tests, and following standard operating procedures.

• Implementing quality control measures such as daily calibration of equipment

• Ensuring accuracy of tests by following proper protocols and techniques

• Following standard operating procedures to maintain consistency and reliability

• Regularly reviewing and updating procedures to meet industry standards

Lymphocytes are a type of white blood cell that play a key role in the immune system.

• Lymphocytes are responsible for recognizing and attacking foreign invaders such as viruses and bacteria.

• There are two main types of lymphocytes: B cells and T cells.

• B cells produce antibodies that can neutralize or destroy pathogens.

• T cells can directly attack infected cells or coordinate the immune response.

• Lymphocytes also play a role in immunological memory, allowing the body to quickly re...read more

To proceed mucoid analysis, I would follow standard protocols and perform QC and calibration regularly. I have worked on multiple chemistry machines.

• I would start by collecting the sample and preparing it for analysis

• Then, I would perform the necessary tests and record the results

• To ensure accuracy, I would perform QC and calibration regularly

• I have experience working on multiple chemistry machines, including XYZ and ABC models

Blood donation is taken by inserting a sterile needle into a vein and collecting blood in a bag.

• Verify donor's eligibility and consent

• Clean the site of insertion with antiseptic

• Insert sterile needle into a vein

• Collect blood in a bag

• Remove needle and apply pressure to the site

• Dispose of the needle and bag properly

Malaria falciparum is more severe and can be fatal, while malaria vivax is less severe but can cause relapses.

• Malaria falciparum is more common in Africa and can cause severe symptoms such as anemia, kidney failure, and cerebral malaria.

• Malaria vivax is more common in Asia and South America and can cause symptoms such as fever, chills, and headache.

• Malaria falciparum can be fatal if not treated promptly, while malaria vivax is rarely fatal but can cause relapses.

• Malaria falci...read more

The question evaluates talent on multiple skill sets and tests teamwork and individual skills, making it ideal for large groups and freshers. Its uniqueness throws candidates out of their set routine.

• Assess candidates' ability to work in a team and individually

• Evaluate their proficiency in multiple skill sets

• Observe how they handle unexpected situations

• Ideal for assessing freshers and large groups

• Examples: group projects, case studies, role-playing scenarios

LFT Test stands for Liver Function Test. It is a blood test that measures the levels of enzymes and proteins in the liver.

• LFT Test is used to assess the overall health and function of the liver.

• It helps in diagnosing liver diseases such as hepatitis, cirrhosis, and fatty liver.

• The test measures various parameters including liver enzymes (AST, ALT), bilirubin, albumin, and alkaline phosphatase.

• Abnormal LFT results may indicate liver damage or dysfunction.

• LFT Test is commonly p...read more

There are three types of vials used for collecting blood samples: serum, plasma, and whole blood.

• Serum vials are used for tests that require a clear, yellowish fluid without clotting factors.

• Plasma vials are used for tests that require clotting factors.

• Whole blood vials are used for tests that require all components of blood, including red and white blood cells.

• Different additives are used in each type of vial to preserve the sample for testing.

I would calmly explain the process of blood donation and address any concerns or misconceptions the attendant may have.

• Listen actively to the attendant's concerns

• Provide clear and concise information about the blood donation process

• Address any misconceptions or fears the attendant may have

• Offer reassurance and support throughout the process

• Ensure the attendant is fully informed and comfortable before proceeding

Cross matching is a process of testing compatibility between donor and recipient blood. There are two types of cross match: immediate spin cross match and full cross match.

• Cross match is done to ensure that the recipient's immune system does not attack the donor's blood cells.

• Immediate spin cross match involves mixing donor and recipient blood and observing for agglutination.

• Full cross match involves testing for compatibility using antigens and antibodies.

• Types of cross match...read more

Yes, I am proficient in blood collection, microscopic examination, and biochemistry.

• I am skilled in blood collection techniques such as venipuncture and capillary puncture.

• I have experience in performing microscopic examinations of various specimens, such as blood smears and urine samples.

• I am knowledgeable in conducting biochemistry tests, interpreting results, and troubleshooting any issues that may arise.

• For example, I have successfully collected blood samples from patient...read more

IMAGE stands for Imaging and Molecular Genetics Expertise. It is a medical term used to describe the use of imaging techniques and genetic analysis in diagnosing and treating diseases.

• IMAGE refers to the combination of imaging techniques (such as X-rays, CT scans, MRI) and molecular genetics analysis.

• It is used in medical laboratories to identify and study genetic abnormalities and diseases.

• IMAGE helps in the diagnosis and monitoring of conditions like cancer, genetic disorde...read more

The order of blood draw is important to ensure accurate results. CBC critical values are important for diagnosing infections and anemia. RFT and LFT parameters help diagnose liver and kidney diseases. Different colored containers are used for different tests.

• Order of blood draw: blood culture, coagulation tests, serum tubes, heparin tubes, EDTA tubes, glycolytic inhibitor tubes

• CBC critical values: hemoglobin, hematocrit, platelet count, white blood cell count

• RFT parameters: c...read more

The first line of defense in the body immune system is the innate immune system.

• Innate immune system is the body's initial response to any foreign invader.

• It includes physical barriers like skin and mucous membranes, as well as non-specific immune cells like neutrophils and macrophages.

• These cells can quickly identify and attack any foreign substance that enters the body.

• Examples of innate immune responses include inflammation, fever, and the production of antimicrobial prote...read more

Methods of sterilization include heat, chemicals, radiation, and filtration.

• Heat sterilization methods include autoclaving, dry heat, and boiling.

• Chemical sterilization methods include using disinfectants and antiseptics.

• Radiation sterilization methods include gamma radiation and ultraviolet light.

• Filtration sterilization methods involve passing substances through filters to remove microorganisms.

• Examples of sterilization techniques include steam sterilization in an autoclave...read more

Blood sample collection via venipuncture involves inserting a needle into a vein to draw blood for testing or donation.

• Select appropriate vein (usually in the arm)

• Clean the site with alcohol swab

• Apply tourniquet to make vein more visible and easier to access

• Insert needle at a slight angle into the vein

• Collect blood into appropriate tubes or containers

• Remove needle and apply pressure to site to stop bleeding

• Label samples correctly for identification

Medical laboratory technicians perform various laboratory tests and procedures to assist in the diagnosis, treatment, and prevention of diseases.

• Collect and analyze body fluids, tissues, and other substances

• Operate and maintain laboratory equipment

• Record and interpret test results

• Communicate with healthcare professionals

• Ensure accuracy and quality control

• Follow safety protocols and maintain a clean work environment

BSL-2 is needed to carry out extraction of infectious agents.

• BSL-2 (Biosafety Level 2) is required for handling infectious agents that pose moderate risk to personnel and the environment.

• BSL-2 facilities have specific requirements for personal protective equipment, engineering controls, and laboratory practices.

• Examples of infectious agents that require BSL-2 include Salmonella, influenza virus, and hepatitis B virus.

To prevent hemolysis of a blood sample, proper handling and collection techniques must be followed.

• Use appropriate needle size and gauge for venipuncture

• Avoid excessive suction during blood collection

• Mix tubes gently after collection to prevent clotting

• Avoid vigorous shaking of tubes

• Ensure tubes are filled to proper volume to maintain blood-to-anticoagulant ratio

The blood will turn brownish yellow after adding n/10 HCl.

• n/10 HCl is a dilute hydrochloric acid solution

• The acid helps to break down the blood cells and release hemoglobin

• The color change is due to the formation of acid hematin

• The test is used to detect the presence of blood in a sample

Blood collection knowledge is essential for a medical laboratory technician.

• Knowledge of different blood collection methods and equipment

• Understanding of proper patient identification and labeling

• Awareness of potential complications and how to handle them

• Familiarity with safety protocols and infection control measures

• Ability to accurately record and document collected specimens

Gram staining is a common laboratory technique used to differentiate bacteria into two major groups: Gram-positive and Gram-negative.

• Gram staining involves a series of steps including fixation, crystal violet staining, iodine treatment, decolorization, and counterstaining with safranin.

• Gram-positive bacteria retain the crystal violet stain and appear purple under a microscope, while Gram-negative bacteria lose the stain and appear pink or red.

• This staining technique is essent...read more

Glycated hemoglobin sample is taken in a purple-topped vial.

• Glycated hemoglobin samples are typically collected in a purple-topped EDTA tube.

• The purple-topped tube contains an anticoagulant to prevent clotting of the blood sample.

• It is important to properly label the tube with patient information and the date and time of collection.

Yes, HIV stands for Human Immunodeficiency Virus.

• HIV is a virus that attacks the immune system, specifically the CD4 cells.

• It can lead to acquired immunodeficiency syndrome (AIDS) if left untreated.

• HIV is primarily transmitted through unprotected sexual intercourse, sharing needles, or from mother to child during childbirth or breastfeeding.

• Testing for HIV involves detecting antibodies or antigens in blood or oral fluid samples.

• Treatment for HIV includes antiretroviral therap...read more

Biochemistry parameters tests in semi auto can be done by following specific steps and protocols.

• Ensure the semi auto analyzer is properly calibrated and maintained

• Prepare the samples according to the manufacturer's instructions

• Load the samples onto the analyzer and start the test

• Monitor the progress of the test and record the results accurately

• Troubleshoot any issues that may arise during the testing process

• Review and verify the results before reporting them

The normal range of platelets is typically between 150,000 and 450,000 platelets per microliter of blood.

• Normal range is 150,000 to 450,000 platelets per microliter of blood

• Platelet count below 150,000 may indicate thrombocytopenia

• Platelet count above 450,000 may indicate thrombocytosis

I have experience working with Roche Integra and Roche e411 machines.

• I am familiar with the operation and maintenance of Roche Integra and Roche e411 machines.

• I have performed various tests using these machines, such as blood chemistry and immunoassays.

• I am able to troubleshoot and resolve issues with these machines to ensure accurate results.

• I am also knowledgeable in interpreting and analyzing the data generated by these machines.