Gram staining procedure
Binal
1y
Prepare smear.
Heat fix the smear.
Then,1st apply the crystal violet on smear for 1min.
Wash the slide with tap water.
Gram Iodine apply on the smear
Then apply the decoloring agent(alcohol)
Applying for th...read more
Anonymous
1y
Smear preper
Hit fix
Crystal vayolet(30sec
D/w
Grams iodin (1min
Acetone(10sec
D/w
Safranin (30sec
Oli emasan 100x observation
Oyesh Ahamad
1y
Slide fixation with N.S. and heat then flood the crystal violet 60 sec. And Iodine treatment 60sec. Then decolourise with alcohol and counter stains with safranine and observe under 100X with oil imme...read more
Anonymous
author
2y
Fixtion slide and iodine treatment and decolourization and counter stain safranin
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