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JHS Svendgaard Laboratories

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Indorama Petrochemical Interview Questions and Answers

Updated 5 Feb 2024

Q1. 3) what do you mean by BET(bacterial endotoxin test)? MVD and it's formula ?

Ans.

BET is a test used to detect bacterial endotoxins in pharmaceutical products. MVD is the maximum valid dilution of a sample.

  • BET is a test performed to ensure the absence of bacterial endotoxins in pharmaceutical products.

  • It is based on the principle of the Limulus amebocyte lysate (LAL) assay.

  • The test involves diluting the sample and adding it to LAL reagent.

  • The MVD is the highest dilution of the sample that does not show any clotting or turbidity.

  • The formula for MVD is MVD =...read more

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Q2. 5) sterilisation and types of sterilisation ?

Ans.

Sterilization is the process of killing or removing all forms of microbial life from an object or environment.

  • Sterilization ensures the complete elimination of all microorganisms, including bacteria, viruses, fungi, and spores.

  • Common methods of sterilization include heat, radiation, filtration, and chemical agents.

  • Heat sterilization methods include autoclaving, dry heat, and flaming.

  • Radiation sterilization can be achieved through gamma radiation or electron beam irradiation.

  • F...read more

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Q3. 1) What is gram staining ?

Ans.

Gram staining is a technique used to differentiate bacteria into two major groups: Gram-positive and Gram-negative.

  • Gram staining involves a series of steps including fixation, staining with crystal violet, iodine treatment, decolorization with alcohol, and counterstaining with safranin.

  • Gram-positive bacteria retain the crystal violet stain and appear purple under a microscope, while Gram-negative bacteria lose the stain and appear pink or red.

  • The staining helps in identifying...read more

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Q4. 6) scope of microbiology ?

Ans.

Microbiology has a vast scope, ranging from the study of microorganisms to their applications in various fields.

  • Study of microorganisms and their characteristics

  • Research on microbial genetics and evolution

  • Development of vaccines and antibiotics

  • Industrial applications such as food and beverage production

  • Environmental applications such as bioremediation

  • Medical applications such as diagnosis and treatment of diseases

  • Exploration of space and extraterrestrial life

  • Research on emerg...read more

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Q5. what is process for product approval by IDA

Ans.

The process for product approval by IDA involves submission of application, review by IDA, inspection, and final decision.

  • The manufacturer submits an application to IDA.

  • IDA reviews the application and conducts an inspection of the manufacturing facility.

  • If the product meets the required standards, IDA grants approval.

  • If the product does not meet the standards, IDA may request additional information or reject the application.

  • The approval process may take several months to comp...read more

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Q6. Do improvement plan

Ans.

To create an improvement plan, identify areas for growth, set specific goals, develop strategies, implement changes, and regularly evaluate progress.

  • Identify areas for improvement based on data analysis or feedback

  • Set specific, measurable, achievable, relevant, and time-bound goals

  • Develop strategies and action plans to achieve the goals

  • Implement changes and monitor progress regularly

  • Adjust the plan as needed based on feedback and results

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