JHS Svendgaard Laboratories
Indorama Petrochemical Interview Questions and Answers
Q1. 3) what do you mean by BET(bacterial endotoxin test)? MVD and it's formula ?
BET is a test used to detect bacterial endotoxins in pharmaceutical products. MVD is the maximum valid dilution of a sample.
BET is a test performed to ensure the absence of bacterial endotoxins in pharmaceutical products.
It is based on the principle of the Limulus amebocyte lysate (LAL) assay.
The test involves diluting the sample and adding it to LAL reagent.
The MVD is the highest dilution of the sample that does not show any clotting or turbidity.
The formula for MVD is MVD =...read more
Q2. 5) sterilisation and types of sterilisation ?
Sterilization is the process of killing or removing all forms of microbial life from an object or environment.
Sterilization ensures the complete elimination of all microorganisms, including bacteria, viruses, fungi, and spores.
Common methods of sterilization include heat, radiation, filtration, and chemical agents.
Heat sterilization methods include autoclaving, dry heat, and flaming.
Radiation sterilization can be achieved through gamma radiation or electron beam irradiation.
F...read more
Q3. 1) What is gram staining ?
Gram staining is a technique used to differentiate bacteria into two major groups: Gram-positive and Gram-negative.
Gram staining involves a series of steps including fixation, staining with crystal violet, iodine treatment, decolorization with alcohol, and counterstaining with safranin.
Gram-positive bacteria retain the crystal violet stain and appear purple under a microscope, while Gram-negative bacteria lose the stain and appear pink or red.
The staining helps in identifying...read more
Q4. 6) scope of microbiology ?
Microbiology has a vast scope, ranging from the study of microorganisms to their applications in various fields.
Study of microorganisms and their characteristics
Research on microbial genetics and evolution
Development of vaccines and antibiotics
Industrial applications such as food and beverage production
Environmental applications such as bioremediation
Medical applications such as diagnosis and treatment of diseases
Exploration of space and extraterrestrial life
Research on emerg...read more
Q5. what is process for product approval by IDA
The process for product approval by IDA involves submission of application, review by IDA, inspection, and final decision.
The manufacturer submits an application to IDA.
IDA reviews the application and conducts an inspection of the manufacturing facility.
If the product meets the required standards, IDA grants approval.
If the product does not meet the standards, IDA may request additional information or reject the application.
The approval process may take several months to comp...read more
Q6. Do improvement plan
To create an improvement plan, identify areas for growth, set specific goals, develop strategies, implement changes, and regularly evaluate progress.
Identify areas for improvement based on data analysis or feedback
Set specific, measurable, achievable, relevant, and time-bound goals
Develop strategies and action plans to achieve the goals
Implement changes and monitor progress regularly
Adjust the plan as needed based on feedback and results
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