Himedia Laboratories Cell Culture Analyst Interview Questions and Answers

Q1. About NGS and Rt PCR
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Q2. Previous experience
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Q1. What is lipid profile
Ans. A lipid profile or lipid panel is a panel of blood tests used to find abnormalities in blood lipid concentrations. The results of this test can identify certain genetic diseases and can determine approximate risks for cardiovascular disease, certain forms of pancreatitis,and other disease
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Q2. How many test of liver
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Q3. The liver function tests typically include alanine transaminase (ALT) and aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), serum bilirubin, prothrombin time (PT),...read more
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Q4. What is normal range of hemoglobin
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Q5. Male. 12 _ 14 mg/dl Female. 10_12mg/dl
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An introductory conversation to determine that you have the basic skills and qualifications for the role and can fit in with the team and company culture

Assignment will be considered by online based on the professional behaviour.

Q1. Related to technical
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Q2. What is HPLC and how it works
Ans. 
HPLC is a high-performance liquid chromatography technique used to separate, identify, and quantify components in a mixture.
• HPLC involves a sample being dissolved in a solvent and passed through a column packed with a stationary phase material.

• Different components in the sample interact differently with the stationary phase, leading to separation based on their properties.

• The separated components are detected and quant...read more
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Q1. General question
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Q1. Process related
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Q1. How to handle and quench pyrophoric chemical in reaction.
Ans. 
Pyrophoric chemicals can be handled and quenched by using inert gases, specialized containers, and proper disposal methods.
• Use inert gases such as nitrogen or argon to displace air and prevent ignition.

• Store and handle pyrophoric chemicals in specialized containers such as air-tight glass ampoules or sealed metal containers.

• Dispose of pyrophoric chemicals properly by contacting a hazardous waste disposal company.

• Exampl...read more
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Q2. Name reactions based on palladium chemistry and copper chemistry
Ans. 
Palladium and copper chemistry are widely used in organic synthesis. Some important reactions include Suzuki coupling, Heck reaction, Sonogashira coupling, and Ullmann reaction.
• Suzuki coupling: cross-coupling reaction between an organoboron compound and an organic halide or triflate catalyzed by a palladium complex

• Heck reaction: coupling of an aryl or vinyl halide with an alkene in the presence of a palladium catalyst

• S...read more
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Q3. Different oxidising and reducing agent for ester reduction,cyano reduction, alchol oxidation
Ans. 
Different oxidising and reducing agents used for ester reduction, cyano reduction, and alcohol oxidation.
• Ester reduction: Lithium aluminum hydride (LiAlH4), sodium borohydride (NaBH4), diisobutylaluminum hydride (DIBAL-H)

• Cyano reduction: Lithium aluminum hydride (LiAlH4), sodium borohydride (NaBH4)

• Alcohol oxidation: Chromium trioxide (CrO3), potassium permanganate (KMnO4), Jones reagent (CrO3/H2SO4)
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Q4. Indole, oxazole and isoxazole synthesis route
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Q1. Check confident level and introduction
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Q2. Work experience and handaling of instrument
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Q3. Knowledge of company product
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Q1. Why are choosing this company
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Q2. Accepted salary and ready to work start
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Q1. Tell me about 5 types of painkiller
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Q2. What should be need to care in industrial area
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