Auro Peptides Interview Questions and Answers

The resins mostly used for solid phase peptide synthesis (SPPS) are polystyrene-based resins.

• Polystyrene-based resins are commonly used due to their high loading capacity and stability during peptide synthesis.

• Other resins like PEG-based resins and TentaGel resins are also used in SPPS for specific applications.

• Polystyrene resins are often cross-linked to improve their mechanical strength and swelling properties.

• Resins...read more

Resin selection for peptides involves considering factors like compatibility, loading capacity, swelling properties, and functional groups.

• Consider the compatibility of the resin with the peptide sequence and protecting groups.

• Evaluate the loading capacity of the resin to ensure efficient peptide synthesis.

• Assess the swelling properties of the resin to facilitate diffusion of reagents and solvents.

• Take into account the...read more

The coupling reagents used in solid-phase peptide synthesis (SPPS) include DIC, HBTU, HATU, and TBTU.

• DIC (diisopropylcarbodiimide) is commonly used as a coupling reagent in SPPS.

• HBTU (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) is another popular coupling reagent.

• HATU (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) is also used in SPPS.

• TBTU (2...read more

Orthogonal protection in solid-phase peptide synthesis (SPPS) involves selectively protecting certain functional groups to prevent unwanted reactions.

• Orthogonal protection allows for stepwise synthesis of peptides by selectively deprotecting and activating specific amino acids while keeping others protected.

• Common protecting groups include Fmoc and Boc for amino groups, and t-butyl for hydroxyl groups.

• Orthogonal protec...read more

I have experience handling various instruments including laboratory equipment, musical instruments, and electronic devices.

• Laboratory equipment such as microscopes, pipettes, and centrifuges

• Musical instruments like guitar, piano, and drums

• Electronic devices including smartphones, laptops, and cameras

Peptide synthesis involves creating peptides through chemical reactions, control strategy ensures quality and consistency, and filling activities involve packaging the peptides into final products.

• Peptide synthesis is the process of creating peptides by joining amino acids together through chemical reactions.

• Control strategy in peptide synthesis involves monitoring reaction conditions, purity of reagents, and product q...read more