Surien Pharmaceuticals QC Chemist Interview Questions and Answers for Experienced

Dos and don'ts in HPLC handling and what to do if sample stopped while running

• Do ensure proper maintenance and calibration of HPLC equipment

• Do use high quality solvents and columns for accurate results

• Don't introduce air bubbles into the system

• Don't overload the column with sample

• If sample stops while running, check for clogs in the column or injector and troubleshoot accordingly

The HPLC should be calibrated before each use to ensure accurate and reliable results.

• HPLC should be calibrated before each use to maintain accuracy

• Calibration frequency may vary depending on the lab's SOPs and manufacturer recommendations

• Regular calibration helps in ensuring reliable results and instrument performance

• Calibration intervals can range from daily to monthly, depending on usage and requirements