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Calibration of GC is a process of adjusting the instrument to ensure accurate and precise measurements.
Calibration should be performed regularly to maintain accuracy.
The process involves comparing the instrument's readings to known standards.
Adjustments are made to the instrument's settings to match the known standards.
Documentation of calibration should be kept for quality control purposes.
Examples of standards used f...
GC troubleshooting involves identifying and resolving issues with the gas chromatography system.
Check for leaks in the system
Verify the column is properly installed and conditioned
Ensure the detector is functioning correctly
Calibrate the instrument regularly
Check for contamination in the sample or column
Inspect the inlet liner and septum for damage or buildup
Review the method parameters for accuracy and precision
I applied via Newspaper Ad and was interviewed in Apr 2022. There were 4 interview rounds.
Top trending discussions
I applied via Recruitment Consulltant and was interviewed in Feb 2022. There were 5 interview rounds.
Gas chromatography is a technique used to separate and analyze volatile compounds in a mixture.
Gas chromatography involves the use of a stationary phase and a mobile phase.
The stationary phase is a solid or liquid coating inside a column, while the mobile phase is a gas.
The mixture is injected into the column, and as the mobile phase flows through, different compounds interact with the stationary phase at different rat...
Buffer solutions with known pH values are used for pH meter calibration.
Buffer solutions with pH values of 4.01, 7.00, and 10.01 are commonly used for pH meter calibration.
The choice of buffer solution depends on the pH range of the sample being tested.
The buffer solutions should be stored properly and replaced regularly to ensure accurate calibration.
Calibration should be performed before each use of the pH meter.
Total productivity Maintenance
Handling of HPLC GC and all other instruments used in Quality control department
I applied via Referral and was interviewed before Dec 2021. There were 2 interview rounds.
I applied via Campus Placement and was interviewed before Jan 2021. There were 2 interview rounds.
Basic test
I applied via Referral and was interviewed in Apr 2023. There were 3 interview rounds.
The length of GC column varies depending on the type and manufacturer.
GC columns can range from 10-100 meters in length.
The length of the column affects the separation of compounds.
Longer columns provide better separation but require more time and may have higher costs.
The length of the column should be chosen based on the specific analysis needs.
Different types of columns may have different optimal lengths.
The length of HPLC column varies depending on the application and type of column used.
The length of HPLC column can range from 30 mm to 300 mm.
Shorter columns are used for faster separations while longer columns are used for higher resolution.
The length of the column can also affect the retention time of the analyte.
For example, a C18 column with a length of 150 mm is commonly used for routine analysis of pharmaceutica
HPLC and GC are both analytical techniques used to separate and analyze compounds, but they differ in their principles and applications.
HPLC stands for High Performance Liquid Chromatography, while GC stands for Gas Chromatography.
HPLC is used for separating and analyzing compounds that are soluble in liquid, while GC is used for separating and analyzing compounds that are volatile and can be vaporized.
HPLC uses a liqu...
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The goal of this company is to provide high-quality products and services to customers.
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I applied via LinkedIn and was interviewed in Jun 2023. There were 2 interview rounds.
Exp, how to working in process line , HAccp , Vaccp , Taccp .
I applied via Walk-in and was interviewed in May 2022. There were 2 interview rounds.
I applied via Walk-in and was interviewed before Mar 2019. There were 3 interview rounds.
Chromatography is a technique used to separate and analyze mixtures of substances based on their different properties.
Chromatography involves a stationary phase and a mobile phase.
The stationary phase is a solid or liquid material that retains the components of the mixture to be separated.
The mobile phase is a liquid or gas that carries the mixture through the stationary phase.
Different components of the mixture intera...
pH is a measure of the acidity or alkalinity of a solution.
pH stands for 'potential of hydrogen'.
It is a logarithmic scale ranging from 0 to 14.
A pH of 7 is considered neutral, below 7 is acidic, and above 7 is alkaline.
Each unit change in pH represents a tenfold change in acidity or alkalinity.
pH is commonly used in various fields such as chemistry, biology, and environmental science.
Nitration reaction is a chemical reaction in which a nitro group (-NO2) is introduced into a molecule.
Nitration is a type of electrophilic aromatic substitution reaction.
It involves the substitution of a hydrogen atom on an aromatic ring with a nitro group.
The reaction is typically carried out using a mixture of nitric acid and sulfuric acid as the nitrating agent.
Nitration reactions are commonly used in the synthesis ...
Karl Fischer reaction is a method for determining the water content in a sample. The instrument used for this reaction is calibrated using standard solutions.
Karl Fischer reaction is a chemical reaction that involves the titration of water with iodine.
It is commonly used in analytical chemistry to measure the moisture content in various substances.
The reaction is based on the principle that iodine reacts with water to ...
Normality refers to the state of being normal or conforming to a standard or norm.
Normality is a concept used in various fields to define what is considered typical or expected.
It is often used to describe the behavior, characteristics, or values that are within the range of what is considered normal.
Normality can vary depending on the context and can be subjective or objective.
In statistics, normality refers to the di...
The main components of HPLC are the mobile phase, stationary phase, pump, injector, column, detector, and data acquisition system.
Mobile phase: The solvent or mixture of solvents that carries the sample through the system.
Stationary phase: The solid or liquid phase that retains the analytes based on their interactions.
Pump: Responsible for delivering the mobile phase at a constant flow rate.
Injector: Introduces the sam...
Samples can be identified based on labeling, appearance, unique characteristics, and accompanying documentation.
Check the labeling on the sample containers for identification information such as sample name, ID number, and date collected.
Examine the physical appearance of the samples, noting color, texture, and any visible impurities.
Look for unique characteristics such as odor or solubility that can help differentiate...
NMR spectroscopy is a technique used to study the structure and composition of molecules by analyzing the magnetic properties of atomic nuclei.
NMR spectroscopy involves applying a magnetic field to a sample, which causes the nuclei of certain atoms to resonate at specific frequencies.
The resulting signals are detected and used to create a spectrum that provides information about the chemical environment of the nuclei.
N...
pH is a measure of acidity or alkalinity of a solution on a scale of 0 to 14.
pH stands for 'potential of Hydrogen'
It measures the concentration of hydrogen ions in a solution
A pH of 7 is considered neutral, below 7 is acidic, and above 7 is alkaline
Examples: Lemon juice has a pH of around 2, while baking soda has a pH of around 9
Ranges in NMR and UV can be identified by analyzing the chemical shifts and absorption peaks, respectively.
In NMR, chemical shifts are measured in parts per million (ppm) and can range from 0 to 12 ppm for proton NMR.
In UV spectroscopy, absorption peaks are observed at specific wavelengths, typically ranging from 200 to 400 nm.
Interpreting the peaks and shifts in the spectra can help determine the functional groups pre
Silica gel is used as a stationary phase in chromatography due to its high surface area and ability to interact with a wide range of compounds.
Silica gel has a high surface area, allowing for better separation of compounds based on their interactions with the stationary phase.
It can interact with a wide range of compounds, making it versatile for different types of chromatography techniques.
Silica gel is chemically ine...
Oxidation is the loss of electrons by a molecule, atom, or ion, while reduction is the gain of electrons.
Oxidation involves the addition of oxygen or loss of hydrogen, while reduction involves the loss of oxygen or gain of hydrogen.
Oxidation increases the oxidation state of a molecule, while reduction decreases it.
An example of oxidation is the rusting of iron, where iron reacts with oxygen to form iron oxide.
An exampl...
Isomerism is a phenomenon in which two or more compounds have the same molecular formula but different structural arrangements.
Isomers have different physical and chemical properties.
Types of isomerism include structural isomerism, stereoisomerism, and conformational isomerism.
Example: Structural isomers - butane and isobutane.
Example: Stereoisomers - cis and trans isomers of 2-butene.
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