SAI Life Sciences Research Chemist Trainee Interview Questions and Answers

LCMS stands for Liquid Chromatography Mass Spectrometry. It is a technique used to separate, identify and quantify components in a mixture.

• LCMS involves the use of a liquid chromatography column to separate the components of a mixture based on their chemical properties.

• The separated components are then ionized and passed through a mass spectrometer to determine their mass-to-charge ratio.

• LCMS is commonly used in drug d...read more

C18 and C4 are commonly used reversed-phase columns in LCMS.

• C18 and C4 refer to the type of stationary phase used in the column.

• C18 has longer carbon chains and is more hydrophobic than C4.

• C18 is commonly used for separating nonpolar compounds while C4 is used for separating polar compounds.

• Other types of columns include C8, phenyl, and cyano columns.

• The choice of column depends on the properties of the analyte and the