Research Chemist Trainee Interview Questions and Answers

C18 and C4 are commonly used reversed-phase columns in LCMS.

• C18 and C4 refer to the type of stationary phase used in the column.

• C18 has longer carbon chains and is more hydrophobic than C4.

• C18 is commonly used for separating nonpolar compounds while C4 is used for separating polar compounds.

• Other types of columns include C8, phenyl, and cyano columns.

• The choice of column depends on the properties of the analyte and the separation goal.

LCMS stands for Liquid Chromatography Mass Spectrometry. It is a technique used to separate, identify and quantify components in a mixture.

• LCMS involves the use of a liquid chromatography column to separate the components of a mixture based on their chemical properties.

• The separated components are then ionized and passed through a mass spectrometer to determine their mass-to-charge ratio.

• LCMS is commonly used in drug discovery, environmental analysis, and forensic science.

• LCM...read more

There are several types of HPLC, including reverse phase, normal phase, ion exchange, size exclusion, and affinity.

• Reverse phase HPLC uses a non-polar stationary phase and a polar mobile phase.

• Normal phase HPLC uses a polar stationary phase and a non-polar mobile phase.

• Ion exchange HPLC separates analytes based on their charge.

• Size exclusion HPLC separates analytes based on their size.

• Affinity HPLC uses a specific binding interaction between analytes and a stationary phase.