Research Chemist Trainee Interview Questions and Answers for Freshers

There are several types of HPLC, including reverse phase, normal phase, ion exchange, size exclusion, and affinity.

• Reverse phase HPLC uses a non-polar stationary phase and a polar mobile phase.

• Normal phase HPLC uses a polar stationary phase and a non-polar mobile phase.

• Ion exchange HPLC separates analytes based on their charge.

• Size exclusion HPLC separates analytes based on their size.

• Affinity HPLC uses a specific binding interaction between analytes and a stationary phase.