Raptakos Brett and Company QA Executive Interview Questions and Answers for Experienced

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Q1. How many parameters of HPLC Calibration ?
Ans. 
There are several parameters for HPLC calibration.
• The parameters include accuracy, precision, linearity, range, limit of detection, and limit of quantification.

• Accuracy refers to how close the measured value is to the true value.

• Precision refers to the reproducibility of results.

• Linearity refers to the ability of the method to produce results that are proportional to the concentration of the analyte.

• Range refers to the...read more
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Q2. What are the calibration parameters of UV Vis Spectrophometer?
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Calibration parameters of UV Vis Spectrophotometer
• Calibration parameters include wavelength accuracy, photometric accuracy, stray light, resolution, and noise.

• Wavelength accuracy is checked using holmium oxide or didymium glass filters.

• Photometric accuracy is checked using neutral density filters.

• Stray light is checked using potassium dichromate or sodium iodide solutions.

• Resolution is checked using holmium oxide or to...read more
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Q3. What is difference between weight of two samples after ignition of ROI?
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Weight difference of two samples after ignition of ROI
• ROI stands for Residue on Ignition

• The weight difference is due to the loss of volatile substances during ignition

• The weight loss can be used to calculate the percentage of volatile substances in the sample

• This technique is commonly used in pharmaceutical and food industries
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Q4. What is IR region range?
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The IR region range refers to the range of wavelengths in the infrared region of the electromagnetic spectrum.
• The IR region range typically spans from approximately 700 nanometers to 1 millimeter.

• It is divided into three main regions: near-infrared (NIR), mid-infrared (MIR), and far-infrared (FIR).

• Each region has different applications and characteristics. For example, NIR is commonly used in remote controls and optica...read more
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Q5. What is the UV range?
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UV range refers to the range of ultraviolet radiation wavelengths.
• UV range is typically divided into three categories: UVA (320-400 nm), UVB (280-320 nm), and UVC (100-280 nm).

• UVA is responsible for skin aging and wrinkling, while UVB causes sunburn and skin cancer.

• UVC is absorbed by the ozone layer and does not reach the Earth's surface.

• UV radiation can also be used for disinfection and sterilization purposes.

• UV range...read more
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