Akhil Systems Interview Questions and Answers

Monoclonal antibodies are produced by a single clone of cells and target a specific antigen, while polyclonal antibodies are produced by multiple clones of cells and target multiple antigens.

• Monoclonal antibodies are derived from a single parent cell, ensuring uniformity in structure and specificity.

• Polyclonal antibodies are produced by multiple different B cells, resulting in a mixture of antibodies with varying specificities.

• Monoclonal antibodies are used in targeted therap...read more

The column in HPLC is where the separation of compounds occurs based on their interactions with the stationary phase.

• The column is packed with a stationary phase material, such as silica or polymer beads

• Compounds in the sample mixture interact differently with the stationary phase, leading to separation

• The mobile phase carries the sample through the column, with compounds eluting at different times based on their interactions

• Column dimensions, particle size, and stationary ph...read more

Types of chromatography include gas chromatography, liquid chromatography, and thin-layer chromatography.

• Gas chromatography separates volatile compounds based on their affinity for a stationary phase

• Liquid chromatography separates compounds based on their interaction with a liquid mobile phase

• Thin-layer chromatography separates compounds on a thin layer of adsorbent material

• Other types include high-performance liquid chromatography (HPLC) and ion exchange chromatography

HPLC is a type of chromatography used for separating compounds in a mixture based on their interactions with a stationary phase and mobile phase.

• HPLC stands for High Performance Liquid Chromatography, while chromatography is a broader term encompassing various techniques for separating mixtures.

• HPLC typically uses a liquid mobile phase, while chromatography can use liquid, gas, or solid phases.

• HPLC is more precise and sensitive than traditional chromatography methods.

• Examples...read more

Formulation is the process of creating a specific mixture or combination of ingredients to achieve a desired outcome.

• Formulation involves selecting and combining ingredients in specific quantities.

• It is commonly used in industries such as pharmaceuticals, cosmetics, and food production.

• Examples include formulating a new medication, creating a skincare product, or developing a new recipe for a food product.

The column used in HPLC is typically a stainless steel tube filled with a stationary phase.

• The column is a crucial component of the HPLC system, where the separation of compounds occurs.

• The stationary phase inside the column interacts with the mobile phase to separate the analytes based on their properties.

• Columns can vary in length, diameter, and particle size depending on the specific application.

• Common types of columns used in HPLC include C18, C8, and silica-based columns...read more

Monoclonal antibodies are identical copies of a single type of antibody that can target specific antigens.

• Produced by cloning a unique white blood cell

• Used in targeted therapy for cancer

• Can be used in diagnostic tests to detect specific proteins