Manvi Biopharma Interview Questions and Answers

Chemical isolation from natural products involves extraction, purification, and characterization techniques.

• Extraction: Use solvents to separate the desired chemical from the natural product.

• Purification: Employ techniques like chromatography or distillation to remove impurities.

• Characterization: Analyze the isolated chemical using spectroscopy or other methods to determine its structure and properties.

Coupling constant is a measure of the interaction between two or more atoms in a molecule.

• It is the distance between the peaks in a nuclear magnetic resonance (NMR) spectrum.

• It provides information about the chemical environment of the atoms in a molecule.

• It is used to determine the connectivity and structure of a molecule.

• Coupling constants can be scalar or dipolar.

• Examples include J-coupling and H-H coupling.