S R Electricals Interview Questions and Answers

Fermentation is a metabolic process that converts sugar to acids, gases, or alcohol. Doubling time is the time taken for a population to double. Microbial growth curve is a graph that shows the growth of microorganisms over time. Filtration is a process of separating solids from liquids or gases using a filter medium.

• Fermentation is used in the production of beer, wine, and bread.

• Doubling time varies depending on the species of bacteria and the environmental conditions.

• Microb...read more

To isolate an organism from a mixed culture, one can use culturing techniques such as pour plates or spread plates and perform Gram staining.

• In pour plate technique, a diluted sample is mixed with agar and poured into a petri dish. In spread plate technique, a small amount of sample is spread over the surface of agar in a petri dish.

• After incubation, individual colonies can be picked and subcultured for further analysis.

• Gram staining can be used to differentiate between Gram-...read more

Enzymes are biological catalysts that speed up chemical reactions. Enzyme kinetics studies their rates and mechanisms of action.

• Enzymes are proteins that catalyze chemical reactions in living organisms.

• They work by lowering the activation energy required for a reaction to occur.

• Enzyme kinetics is the study of how enzymes work, including their rates and mechanisms of action.

• Enzyme inhibition is the process by which an enzyme's activity is reduced or stopped by a molecule that ...read more

Media optimization is the process of improving the performance of media campaigns by adjusting various parameters. Media preparation is the process of creating and organizing media assets for use in campaigns.

• Media optimization involves adjusting parameters such as targeting, ad format, and bidding strategy to improve campaign performance.

• Media preparation involves creating and organizing media assets such as images, videos, and ad copy for use in campaigns.

• Examples of media ...read more

HPLC is a high-performance liquid chromatography used to separate, identify, and quantify components in a mixture.

• HPLC uses a liquid mobile phase and a solid stationary phase to separate components based on their chemical properties.

• Types of detectors include UV-Vis, fluorescence, electrochemical, and mass spectrometry.

• TLC is a thin-layer chromatography that uses a solid stationary phase and a liquid mobile phase.

• TLC is less sensitive and less accurate than HPLC.

• HPLC is commo...read more

Western blotting is named after Southern blotting, a similar technique named after its inventor, Edwin Southern.

• Western blotting is a technique used to detect specific proteins in a sample.

• It involves separating proteins by size using gel electrophoresis and then transferring them to a membrane.

• The membrane is then probed with a specific antibody that binds to the protein of interest.

• The name 'Western' was coined as a play on words, since it followed the same principles as So...read more

Nutrient media contains carbohydrates, amino acids, vitamins, and minerals to support microbial growth. Examples of microorganisms include E. coli and S. aureus.

• Nutrient media is a general-purpose media that supports the growth of a wide range of microorganisms.

• It contains carbohydrates, amino acids, vitamins, and minerals to provide essential nutrients for microbial growth.

• Examples of microorganisms that can grow on nutrient media include E. coli, S. aureus, and Bacillus sub...read more

DNA isolation is the process of extracting DNA from cells or tissues.

• It involves breaking open the cells and separating the DNA from other cellular components.

• Various methods are used for DNA isolation, including organic extraction, column-based purification, and magnetic bead-based purification.

• DNA isolation is a crucial step in many applications, such as PCR, sequencing, and genetic engineering.

• Examples of DNA isolation kits include Qiagen DNeasy, Promega Wizard, and Thermo...read more