Biocon Limited Principal Scientist Interview Questions and Answers

Q1. Qc microbiology specialization
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Q1. Current CTC and notice period
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I am currently earning a CTC of X and my notice period is Y.
• My current CTC is X.

• My notice period is Y.
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Q1. Stability aspects of proteins
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Stability of proteins is crucial for their function and activity.
• Proteins can denature or unfold due to changes in temperature, pH, or chemical environment.

• Stabilizing factors include disulfide bonds, hydrophobic interactions, and salt bridges.

• Chaperone proteins can assist in protein folding and prevent misfolding.

• Protein stability can be assessed through techniques such as circular dichroism and differential scanning ...read more
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Q1. Factor affecting the lyophilization
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Factors affecting lyophilization
• Temperature: The temperature during the lyophilization process affects the rate of sublimation and drying.

• Pressure: The pressure inside the lyophilizer chamber affects the rate of sublimation and drying.

• Product formulation: The composition and concentration of the product being lyophilized can impact the process.

• Freezing method: The method used to freeze the product prior to lyophilizati...read more
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Q2. What is the meaning of Triple point
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The triple point is the temperature and pressure at which all three phases of a substance (solid, liquid, and gas) coexist in equilibrium.
• The triple point is a unique combination of temperature and pressure for a substance.

• At the triple point, the solid, liquid, and gas phases of the substance can coexist in equilibrium.

• It is the point on a phase diagram where all three phases meet.

• The triple point is a fixed point use...read more
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Q3. Validation of lyophilizer
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Validation of lyophilizer involves testing and documentation to ensure its performance and reliability.
• Validation of lyophilizer is necessary to ensure that it consistently produces high-quality freeze-dried products.

• The process involves testing various parameters such as temperature, pressure, and drying time to ensure they meet predetermined specifications.

• Documentation of the validation process is crucial for regula...read more
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Q4. How to maintain HSO in lyophilized product
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Maintaining HSO in lyophilized product involves proper storage conditions and packaging.
• Store the lyophilized product in a cool and dry environment to prevent moisture absorption.

• Use appropriate packaging materials that provide a barrier against moisture and oxygen.

• Ensure the lyophilized product is sealed tightly to prevent any exposure to air.

• Consider using desiccants or moisture-absorbing packets in the packaging.

• Per...read more
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Q1. About Job Profile
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Q1. About experience
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Q1. Negotiation of salary
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An introductory conversation to determine that you have the basic skills and qualifications for the role and can fit in with the team and company culture

Q1. Tell me about 5 types of painkiller
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Q2. What should be need to care in industrial area
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Q1. Be thorough with all the details mentioned in the resume
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Q1. What is post translational modification
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Post translational modification refers to the chemical modification of a protein after it has been synthesized.
• Common modifications include phosphorylation, glycosylation, and acetylation.

• These modifications can alter the protein's function, stability, and localization.

• Examples of post translational modification include the addition of a phosphate group to a serine or threonine residue, or the addition of a sugar molec...read more
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Q2. Describe your previous role
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Q1. How do you decide which genes to knock out
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Q2. What softwares do you know or have used for analyzing data
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I have used various softwares for data analysis including R, Python, Excel, and SPSS.
• R

• Python

• Excel

• SPSS
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Q1. What are your strengths and weaknesses?
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Q2. Tell me about yourself.
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