Acess Meditech Interview Questions and Answers

Assay by HPLC is a technique used to separate, identify, and quantify components in a sample.

• HPLC stands for High-Performance Liquid Chromatography.

• It is commonly used in pharmaceutical and chemical industries for quality control purposes.

• Assay by HPLC involves injecting a sample into a high-pressure liquid chromatography system, where the components are separated based on their interactions with the stationary phase.

• The separated components are then detected and quantified u...read more

To prepare a 1.0 N NaOH solution, dissolve 40.0 g of NaOH in enough water to make 1 liter of solution.

• To prepare a 1.0 N NaOH solution, you need to know the molar mass of NaOH (40.0 g/mol).

• Calculate the number of moles of NaOH required using the formula: moles = N x volume (in liters).

• For a 1.0 N solution, the number of moles of NaOH is equal to the desired concentration (1.0 mol/L).

• Weigh 40.0 g of NaOH using a balance.

• Dissolve the weighed NaOH in a small amount of water to m...read more

The molecular weight of HNO3 is 63.01 g/mol.

• The molecular weight of a compound is the sum of the atomic weights of all the atoms in its chemical formula.

• For HNO3, the atomic weight of hydrogen (H) is 1.01 g/mol, nitrogen (N) is 14.01 g/mol, and oxygen (O) is 16.00 g/mol.

• Multiplying the atomic weights by the number of atoms in the formula (1 hydrogen, 1 nitrogen, and 3 oxygen) and summing them gives the molecular weight of HNO3 as 63.01 g/mol.

The molecular weight of acetic acid is 60.052 g/mol.

• The molecular weight of a compound is the sum of the atomic weights of all the atoms in its chemical formula.

• The chemical formula of acetic acid is CH3COOH.

• The atomic weights of carbon (C), hydrogen (H), and oxygen (O) are 12.01 g/mol, 1.008 g/mol, and 16.00 g/mol respectively.

• Calculating the molecular weight of acetic acid: (12.01 * 2) + (1.008 * 4) + 16.00 + 1.008 = 60.052 g/mol.

Calibration of Karl Fischer is a process to ensure accuracy and reliability of the moisture determination method.

• Calibration involves verifying the performance of the Karl Fischer titration system.

• It includes checking the accuracy of the reagents, electrodes, and the instrument itself.

• Calibration is typically done using standard solutions with known moisture content.

• The instrument readings are compared to the expected values to determine any deviations.

• Adjustments are made to...read more

R. S in HPLC stands for Relative Standard Deviation.

• R. S is a measure of the precision or reproducibility of an HPLC method.

• It is calculated by dividing the standard deviation of peak areas by the average peak area and multiplying by 100.

• R. S is expressed as a percentage and indicates the variability of peak areas in replicate injections.

• A lower R. S value indicates higher precision and better method performance.

• For example, if the R. S value is 2%, it means that the peak are...read more

Calibration of HPLC involves verifying the accuracy and precision of the instrument by comparing the results obtained with known standards.

• Calibration of HPLC is essential to ensure accurate and reliable results.

• It involves preparing a series of standard solutions with known concentrations of analytes.

• These standard solutions are then injected into the HPLC system and the peak areas or heights are measured.

• The obtained data is used to create a calibration curve, which relates...read more