Acme Formulation Quality Control Analyst Interview Questions and Answers

HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate, identify, and quantify components in a mixture.

• HPLC involves a sample being dissolved in a solvent and then pumped through a column packed with a stationary phase.

• Different components in the sample interact differently with the stationary phase, leading to separation based on their properties.

• The separated components are detecte...read more

Various types of dissolution include intrinsic dissolution, dissolution rate, and dissolution efficiency.

• Intrinsic dissolution: measures the dissolution rate of a pure substance under standardized conditions.

• Dissolution rate: measures the amount of substance that dissolves over time.

• Dissolution efficiency: measures the extent to which a substance dissolves in a given solvent.

• Other types include surface dissolution, bul...read more

Calibration of HPLC involves preparing standard solutions, running them through the system, adjusting parameters, and verifying results.

• Prepare standard solutions of known concentrations.

• Run the standard solutions through the HPLC system.

• Adjust parameters such as flow rate, column temperature, and detector wavelength.

• Verify the results by comparing the peak areas or retention times with known standards.

• Document the cal