Thermo Fisher Scientific Senior Manufacturing Technician Interview Questions and Answers

The interview questions were related to reactor design, fermentation, and impeller power number.

• Power number is a dimensionless number used to describe the power consumption of an impeller in a reactor.

• Fermentation is a process in which microorganisms are used to produce a desired product, such as ethanol or antibiotics.

• Biocon is a leading biopharmaceutical company that specializes in the development and manufacture of...read more

Process flow is the sequence of steps involved in the production of a product or service.

• Process flow outlines the steps required to complete a task or project

• It helps to identify bottlenecks and areas for improvement

• Examples include manufacturing processes, software development, and customer service workflows

Protein binding to resin is a process where proteins are immobilized onto a solid support matrix.

• Resin is typically made of a porous material such as agarose or cellulose beads

• Proteins bind to the resin through various interactions such as hydrogen bonding, electrostatic interactions, and hydrophobic interactions

• This process is commonly used in protein purification and chromatography techniques

• The choice of resin and b...read more

Isoelectric point is the pH at which a molecule has no net charge.

• It is the pH at which a molecule has equal number of positive and negative charges.

• It is also known as the pI.

• At the isoelectric point, a molecule will not migrate in an electric field.

• It is important in protein purification and separation techniques.

Ultrafiltration is a process of separating molecules based on their size using a semipermeable membrane. Diafiltration is a process of washing the separated molecules to remove impurities.

• Ultrafiltration is used in industries such as food and beverage, pharmaceuticals, and biotechnology to purify and concentrate solutions.

• Diafiltration is commonly used in protein purification to remove salts and other impurities from t...read more

AWFI can be qualified through testing and certification processes.

• AWFI can be qualified through third-party testing and certification processes

• Testing may include performance, safety, and environmental standards

• Certification may be required by regulatory bodies or industry associations

• Examples of AWFI certification programs include UL, CE, and ISO

• Qualification may also involve audits of manufacturing processes and qual

Flow through collection is a process of continuous sample collection while fraction collection is the process of collecting specific portions of a sample.

• Flow through collection involves collecting a continuous stream of sample without interruption

• Fraction collection involves collecting specific portions of a sample based on predetermined criteria

• Both processes are commonly used in chromatography and other analytical t...read more

CEX Fraction pooling is done based on the desired purity and yield of the final product.

• CEX Fraction pooling is a process used in protein purification.

• It involves combining multiple fractions from a cation exchange chromatography column.

• The fractions are pooled based on the desired purity and yield of the final product.

• Factors such as protein concentration, pH, and buffer composition are also considered.

• CEX Fraction po...read more

The overall pH and conductivity of the DS depend on the specific DS being referred to.

• The pH and conductivity of different DS can vary widely depending on their composition and intended use.

• For example, the pH of a cleaning solution DS may be around 10-11, while the pH of a battery electrolyte DS may be around 1-2.

• Similarly, the conductivity of a DS used in electronics manufacturing may be very low, while the conductiv...read more

The molecular weight of the target protein is unknown.

• The molecular weight of a protein is the sum of the atomic weights of all the atoms in the protein molecule.

• It is an important parameter for protein purification and characterization.

• The molecular weight can be determined using techniques such as mass spectrometry or gel electrophoresis.

NWP stands for Normalized Water Permeability in Tangential Flow Filtration (TFF).

• NWP is a measure of the water permeability of a TFF membrane normalized by its surface area and thickness.

• It is used to evaluate the performance of TFF membranes in terms of their ability to retain solutes while allowing water to pass through.

• Higher NWP values indicate better membrane performance.

• NWP is affected by factors such as membrane...read more