Career Upcrest Interview Questions and Answers

Peak shape in melting range can be broad or sharp depending on factors like sample purity, heating rate, and instrument calibration.

• Broad peak shape can indicate impurities or a mixture of compounds in the sample.

• Sharp peak shape suggests a pure compound with a narrow melting range.

• Heating rate affects peak shape, with slower rates often resulting in sharper peaks.

• Instrument calibration and resolution can also impact peak shape.

• Example: A sample containing impurities may exhi...read more

The columns used during compositional accuracy of HPLC calibration are known as reference standards.

• Reference standards are used to ensure the accuracy and precision of HPLC calibration.

• These standards are typically composed of known compounds that are used to calibrate the HPLC system.

• The reference standards are run through the HPLC system and the resulting data is compared to the expected values.

• If the results are within an acceptable range, the HPLC system is considered to...read more

Caffeine is used in HPLC calibration as a reference compound due to its unique properties.

• Caffeine has a well-defined retention time and UV absorbance spectrum, making it a reliable reference compound.

• It is also non-toxic and easily available, making it a convenient choice for calibration.

• Caffeine is used to determine the retention time, peak shape, and resolution of the HPLC system.

• It is also used to check the accuracy and precision of the HPLC method.

• Caffeine is added to th...read more

Acetone is used in HPLC calibration as a solvent and to remove impurities.

• Acetone is a common solvent used in HPLC calibration

• It is used to dissolve the sample and standards

• Acetone is also used to remove impurities from the HPLC system

• It is a volatile solvent and evaporates quickly

• Acetone is compatible with most HPLC columns and detectors

Answering questions related to HPLC, impurities, force degradation, solvent classification, and reaction monitoring.

• If a column is fitted in the opposite direction in HPLC, it can lead to inaccurate results.

• Impurities can be classified as organic, inorganic, or residual solvents.

• Force degradation is the process of intentionally subjecting a drug substance to stress conditions to evaluate its stability.

• Other methods for reaction monitoring include UV-Vis spectroscopy, NMR spec...read more

The parameter of Gas Chromatography refers to the variable being measured or analyzed in the process.

• The parameter can be the retention time of the analyte, the peak area, or the peak height.

• It can also be the resolution between two peaks or the selectivity factor.

• The parameter is important in determining the identity and quantity of the analyte in the sample.

• It can also be used to optimize the separation conditions for better resolution and sensitivity.