Spc Life Sciences Executive Interview Questions and Answers

A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.

• pH meter measures the concentration of hydrogen ions in a solution

• Calibration involves adjusting the pH meter to known standard solutions

• Regular calibration ensures accurate readings over time

HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.

• HPLC involves a pump to push the liquid solvent through the system.

• It includes an injector to introduce the sample into the mobile phase.

• The column separates the components of the sample based on their interactions with the stationary phase.

• A detector measures the amount of analyte in the eluent.

• ...read more

Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.

• Phosphate buffers are commonly used in HPLC for separating acidic compounds.

• Acetate buffers are used for separating basic compounds.

• Citrate buffers are used for separating metal ions.

• Other common buffers used in HPLC include ammonium acetate and ammonium formate.

HPLC calibration involves adjusting parameters to ensure accurate and reliable results.

• Prepare standard solutions of known concentrations

• Run the standard solutions through the HPLC system

• Compare the results to expected values and adjust parameters as needed

• Repeat the process until accurate and consistent results are obtained

Handling qualification and operations equipment is crucial for ensuring compliance and efficiency in various industries.

• Calibration equipment: Used to ensure accuracy of measuring instruments, e.g., pressure gauges.

• Testing devices: Employed for quality assurance, such as tensile testing machines in manufacturing.

• Safety equipment: Includes personal protective equipment (PPE) like gloves and helmets in construction.

• Produ...read more

I have experience troubleshooting various equipment by identifying issues, analyzing root causes, and implementing solutions.

• Identifying common issues with the equipment

• Analyzing root causes of malfunctions

• Implementing solutions to resolve problems

• Regular maintenance to prevent future issues

Troubleshooting of HPLC involves identifying and resolving issues with the high-performance liquid chromatography system.

• Check for leaks in the system and tighten connections if necessary.

• Inspect and clean the columns regularly to ensure optimal performance.

• Calibrate the detector and pump to maintain accuracy of results.

• Troubleshoot error messages on the HPLC software by checking system settings and parameters.

• Consult ...read more

Caffeine is used for calibration of HPLC due to its well-defined retention time and peak shape.

• Caffeine has a well-defined retention time, making it easy to identify and calibrate the HPLC system.

• Caffeine produces a sharp and symmetrical peak, which helps in accurately determining the system's performance.

• It is a commonly used compound for calibration in HPLC due to its stability and reproducibility.

• Using caffeine as a...read more

Automatic visual inspection machines use computer vision technology to detect defects in products.

• AVI machines use cameras and software to analyze products for defects

• They can detect defects such as scratches, dents, and discoloration

• AVI machines are commonly used in industries such as manufacturing and packaging

• They can improve quality control and reduce the need for manual inspection

Chemical name and formula are identifiers for specific chemical compounds.

• Chemical name is the common name given to a chemical compound.

• Chemical formula represents the elements and their ratios in a compound.

• Example: Water - Chemical name, H2O - Chemical formula.

To make 0.1M HCL, dilute concentrated hydrochloric acid with water.

• Dilute 1 part of concentrated HCL with 9 parts of water to make 0.1M HCL solution.

• Always add acid to water slowly to prevent splashing and heat generation.

• Use proper protective equipment such as gloves and goggles when handling acids.