Sarv Bio Lab Interview Questions, Process, and Tips

The C=O group gives a peak in the 13CNMR spectrum between 160-220 ppm.

• The peak is typically observed between 160-220 ppm.

• The exact chemical shift depends on the nature of the carbonyl group and its neighboring atoms.

• The peak can be used to identify the presence of a carbonyl group in a molecule.

• Examples of carbonyl-containing functional groups include ketones, aldehydes, carboxylic acids, and esters.

Presence of Br can be identified by observing a peak at m/z value corresponding to the isotope of Br.

• Look for a peak at m/z value corresponding to the isotope of Br (79Br or 81Br)

• Check for the presence of characteristic fragments such as [M-Br]+ or [M-2Br]+

• Compare the mass spectra of the compound with and without Br to identify the difference

• Use other spectroscopic techniques such as NMR to confirm the presence of Br

Nuclear Overhauser effect is a phenomenon in NMR spectroscopy where nuclear spins of different atoms interact with each other.

• It is used to determine the distance between two atoms in a molecule.

• It occurs due to the transfer of nuclear spin polarization from one nucleus to another.

• It is more pronounced when the two nuclei are close in space.

• It is commonly used in structural biology to study protein structures.

• It can al...read more

The Grignard reaction involves the addition of an organomagnesium halide to a carbonyl group, resulting in the formation of a new carbon-carbon bond.

• Named after French chemist Victor Grignard

• Used to synthesize alcohols, carboxylic acids, and other compounds

• Requires anhydrous conditions and a non-protic solvent

• Example: Addition of phenylmagnesium bromide to formaldehyde results in the formation of benzyl alcohol