Navitas Life Sciences Scientist Interview Questions and Answers

Aptitude. English and Quantitative Aptitude

Q1. Basics of flowcytometry
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Q1. Discussion regarding salary structure
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Q1. Presentation of your research work
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Q1. What's ESI and APCI ?
Ans. 
ESI and APCI are ionization techniques used in mass spectrometry.
• ESI stands for Electrospray Ionization and is used for analyzing polar and charged molecules.

• APCI stands for Atmospheric Pressure Chemical Ionization and is used for analyzing non-polar and neutral molecules.

• Both techniques involve ionizing the sample molecules to create ions that can be analyzed by mass spectrometry.

• ESI is commonly used in proteomics and...read more
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Q2. How do you will select ionisation mode ?
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The ionisation mode is selected based on the type of sample and the information required from the analysis.
• Consider the polarity of the sample and the analyte of interest

• Determine the type of information required from the analysis

• Evaluate the sensitivity and selectivity of different ionisation modes

• Examples: ESI for polar compounds, APCI for non-polar compounds, MALDI for large biomolecules
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Q1. CIS and trance proton pattern in NMR
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CIS and trance proton pattern in NMR refers to the orientation of protons in a molecule.
• CIS refers to the protons that are on the same side of a double bond or ring in a molecule

• Trans refers to the protons that are on opposite sides of a double bond or ring in a molecule

• The pattern of these protons can be observed in NMR spectroscopy and can provide information about the structure of the molecule

• For example, in a molec...read more
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Q2. Oxidation of alcohol to acid
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Oxidation of alcohol to acid is a chemical reaction that involves the loss of electrons from an alcohol molecule resulting in the formation of a carboxylic acid.
• The oxidation of primary alcohols results in the formation of aldehydes which are further oxidized to carboxylic acids.

• Secondary alcohols are oxidized to ketones which cannot be further oxidized.

• Tertiary alcohols cannot be oxidized to carboxylic acids.

• Common ox...read more
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Q1. Basic technical questions were asked.
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Q2. Buffer preparation was asked
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Q3. DNA base pairing details were asked
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Questions on attitude, interest and team work etc were asked

Q1. Some basic questions of excel, SQL and Python. Focused more on projects mentioned in CV
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How much you rate in Python and SQL

Q1. Basic details about my previous work profile and technical discussion
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Q1. Explain the machanism of cross coupling reaction
Ans. 
Cross coupling reaction involves the formation of a covalent bond between two different molecules or functional groups.
• It is a type of reaction used in organic chemistry to form carbon-carbon, carbon-nitrogen, or carbon-oxygen bonds.

• It involves the use of a catalyst, typically a transition metal, to facilitate the reaction.

• Examples include the Suzuki-Miyaura reaction, the Heck reaction, and the Stille reaction.

• Cross co...read more
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Q2. What is the use of HATU in acid amine coupling?
Ans. 
HATU is used as a coupling reagent in acid amine coupling to form peptide bonds.
• HATU stands for 2-(1H-7-Azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate

• It is a highly efficient coupling reagent used in solid-phase peptide synthesis

• HATU activates carboxylic acids to form reactive intermediates that can react with amines to form peptide bonds

• It is preferred over other coupling reagents like DCC and DI...read more
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