Mehta Api QC Chemist Interview Questions and Answers

Calibration process for UV and HPLC instruments involves setting up reference standards, adjusting parameters, and verifying accuracy.

• Prepare reference standards for UV and HPLC analysis

• Adjust wavelength, detector sensitivity, and flow rate for UV instrument

• Run standard solutions to verify accuracy and make necessary adjustments

• Perform system suitability tests to ensure instrument performance meets requirements

Reverse phase chromatography uses a non-polar stationary phase and a polar mobile phase, while normal phase chromatography uses a polar stationary phase and a non-polar mobile phase.

• Reverse phase chromatography is more commonly used in analytical chemistry for separating non-polar compounds, while normal phase chromatography is used for polar compounds.

• In reverse phase chromatography, the more non-polar compounds elute...read more

HPLC stands for High Performance Liquid Chromatography, a technique used to separate, identify, and quantify components in a mixture.

• HPLC works on the principle of separating components in a mixture based on their interaction with a stationary phase and a mobile phase.

• The stationary phase is typically a column packed with a solid material, while the mobile phase is a liquid solvent that carries the sample through the c...read more

GLP stands for Good Laboratory Practice, a set of principles intended to ensure the quality and integrity of non-clinical laboratory studies.

• GLP is a quality system concerned with the organizational process and conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, archived, and reported.

• It ensures that the data generated in such studies are reliable and...read more

Acids are substances that donate hydrogen ions, while bases are substances that accept hydrogen ions.

• Acids have a pH less than 7, while bases have a pH greater than 7.

• Acids taste sour, while bases taste bitter.

• Acids react with metals to produce hydrogen gas, while bases do not.

• Examples of acids include hydrochloric acid and sulfuric acid, while examples of bases include sodium hydroxide and ammonia.

• Acids and bases can