Glenmark Pharmaceuticals Clinical Research Associate Interview Questions and Answers for Experienced

Mass spectrometry is a technique used to identify and quantify molecules based on their mass-to-charge ratio.

• Electrospray ionization (ESI)

• Matrix-assisted laser desorption/ionization (MALDI)

• Gas chromatography-mass spectrometry (GC-MS)

• Liquid chromatography-mass spectrometry (LC-MS)

• Time-of-flight mass spectrometry (TOF-MS)

UV spectrometry is a technique that measures the absorption or transmission of ultraviolet light by a sample.

• UV spectrometry is based on the principle that different molecules absorb UV light at specific wavelengths.

• It is commonly used in analytical chemistry to determine the concentration of a substance in a sample.

• UV spectrometry can be used to analyze the purity of a compound or identify unknown substances.

• The instr...read more

To prepare 0.1 N HCl and NaOH, you need to calculate the required amount of the respective chemicals and dilute them in water.

• Calculate the molar mass of HCl and NaOH

• Determine the volume of solution needed

• Use the formula: Normality (N) = (Number of equivalents / Volume of solution in liters)

• For 0.1 N HCl, calculate the number of equivalents of HCl and dilute it to the desired volume

• For NaOH, calculate the number of equ...read more

Quality assurance is the process of ensuring that products or services meet the desired level of quality.

• It involves establishing standards and procedures to ensure that products or services meet those standards.

• It includes monitoring and evaluating the quality of products or services to identify areas for improvement.

• It also involves taking corrective action when necessary to address quality issues.

• Examples include co...read more

Recall definitions

• Recall: the ability to retrieve information from memory

• Definition: a statement that explains the meaning of a word or phrase

• Research: the systematic investigation into and study of materials and sources in order to establish facts and reach new conclusions

• Associate: a person who works with others towards a common goal

• Hypothesis: a proposed explanation for a phenomenon

• Experiment: a scientific procedure...read more

The type of HPLC used depends on the specific research requirements and objectives.

• There are several types of HPLC systems available, including reverse phase, normal phase, ion exchange, size exclusion, and affinity chromatography.

• The choice of HPLC system depends on the properties of the analyte, such as polarity, size, charge, and affinity.

• For example, reverse phase HPLC is commonly used for the separation of nonpola...read more

UV detectors and HPLC detectors are used in research to analyze and quantify substances.

• UV detectors are commonly used in UV-visible spectroscopy to measure the absorption of light by a sample at different wavelengths.

• HPLC detectors are used in high-performance liquid chromatography to detect and quantify the components of a mixture.

• Common types of UV detectors include diode array detectors (DAD) and photodiode array d...read more

Matrix in MALDI MS system is a substance used to assist in ionization of the sample for analysis.

• Matrix is a substance that absorbs energy from the laser and transfers it to the sample, causing ionization.

• The choice of matrix depends on the type of sample being analyzed.

• Common matrices include alpha-cyano-4-hydroxycinnamic acid (CHCA) and sinapinic acid (SA).

Seeking new challenges and opportunities for growth.

• Desire for new challenges and opportunities for growth

• Seeking a more stimulating work environment

• Looking to expand skill set and knowledge

• Company restructuring or downsizing

• Relocation to a new area

Shake flask study is a method used to evaluate the growth and productivity of microorganisms in a small-scale culture.

• It involves growing microorganisms in a small flask with shaking to provide oxygen and nutrients.

• The flask is incubated at a specific temperature and pH to mimic the conditions of the larger culture.

• Samples are taken at regular intervals to measure cell growth and product formation.

• Shake flask studies a...read more

Glycerol is used for long term preservation of bacterial cultures due to its ability to prevent ice crystal formation and cell damage.

• Glycerol acts as a cryoprotectant, preventing ice crystal formation and cell damage during freezing.

• It allows for long term storage of bacterial cultures at -80°C.

• Glycerol is added to the bacterial culture before freezing to a final concentration of 15-20%.

• It is a common practice in micr...read more

The two most important reactions for humans, animals and plants are photosynthesis and respiration.

• Photosynthesis is the process by which plants convert sunlight, carbon dioxide and water into glucose and oxygen.

• Respiration is the process by which living organisms convert glucose and oxygen into energy, carbon dioxide and water.

• Photosynthesis and respiration are complementary processes that sustain life on Earth.

• Photos...read more

My 12th marks were affected due to personal reasons, but I have consistently performed well in other academic pursuits.

• I faced some personal challenges during my 12th grade which affected my performance.

• However, I have consistently performed well in other academic pursuits such as my graduation.

• My 10th grade marks are a reflection of my academic potential and I have worked hard to maintain that level of performance.

• I h...read more

HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate, identify, and quantify components in a mixture.

• HPLC is a widely used analytical technique in chemistry and biochemistry.

• It involves the use of a liquid mobile phase and a solid stationary phase to separate the components of a mixture based on their interactions with the stationary phase.

• The components are separated based on thei...read more

Zetasizer measures particle size and zeta potential while Mastersizer measures particle size distribution.

• Zetasizer uses dynamic light scattering to measure particle size and zeta potential.

• Mastersizer uses laser diffraction to measure particle size distribution.

• Zetasizer is used for colloidal systems while Mastersizer is used for dry powders and granules.

• Zetasizer can measure particles in the range of 0.3 nm to 10 mic...read more

HPLC and UPLC are both chromatography techniques used for separation and analysis of compounds, but differ in their operating pressures and particle sizes.

• HPLC uses larger particle sizes and operates at lower pressures than UPLC.

• UPLC uses smaller particle sizes and operates at higher pressures than HPLC.

• UPLC provides higher resolution and faster separations than HPLC.

• HPLC is more commonly used for routine analysis, whi...read more