Biocon Biologics Chemist Interview Questions and Answers

UV-Vis spectroscopy is a technique that measures the absorption of ultraviolet and visible light by a sample.

• UV-Vis spectroscopy is used to determine the concentration of a sample, as well as its purity and identity.

• It works by passing light through a sample and measuring the amount of light absorbed at different wavelengths.

• The resulting spectrum can be used to identify functional groups and chemical bonds in the samp...read more

pH range for solutions is a measure of acidity or alkalinity on a scale of 0-14.

• A pH of 7 is neutral, below 7 is acidic, and above 7 is alkaline.

• Acidic solutions have a pH below 7, such as lemon juice (pH 2) or vinegar (pH 3).

• Alkaline solutions have a pH above 7, such as baking soda (pH 9) or bleach (pH 12).

• The pH range is important in many fields, including chemistry, biology, and environmental science.

KF autotitrator is an instrument used for determining water content in a sample by titration with Karl Fischer reagent.

• KF autotitrator uses Karl Fischer reagent to determine water content in a sample

• It is an automated titration instrument

• The principle behind KF autotitrator is based on the reaction between iodine and sulfur dioxide in the presence of water

• The instrument measures the amount of reagent required to reach ...read more

HPLC is a technique used to separate, identify, and quantify components in a mixture. There are various types of chromatography techniques.

• HPLC stands for High Performance Liquid Chromatography

• It involves the separation of components in a mixture based on their interaction with a stationary phase and a mobile phase

• Other types of chromatography include gas chromatography, ion exchange chromatography, and size exclusion ...read more

Volumetric solutions are prepared by accurately measuring the volume of a solute and dissolving it in a solvent to a known volume.

• Volumetric solutions are used in analytical chemistry for titrations and other quantitative analyses.

• The solute is usually a solid or liquid that is weighed on a balance before being added to the solvent.

• The solvent is typically water, but other solvents may be used depending on the solute b...read more

Potentiometry is a method of measuring the potential difference between two electrodes in a solution to determine the concentration of an ion.

• Potentiometry is based on the principle that the potential difference between two electrodes in a solution is proportional to the concentration of an ion.

• It is commonly used in analytical chemistry to measure the concentration of ions in a solution.

• Potentiometry can be used to me...read more

Volumetric titrations are of different types based on the nature of the reaction involved.

• Acid-base titration

• Redox titration

• Complexometric titration

• Precipitation titration

• Non-aqueous titration