Molecular Biologist Interview Questions and Answers

Answers to questions related to RT-PCR, biosafety level, and controls for validation.

• The basic principle of RT-PCR is to amplify and detect RNA sequences using reverse transcription and polymerase chain reaction.

• The master mix for RT-PCR typically includes primers, Taq polymerase, dNTPs, buffer, and RNA template.

• Donning and doffing are procedures for putting on and taking off personal protective equipment (PPE) to prevent contamination.

• The biosafety level required to handle i...read more

Molecular biologists study the processes of DNA replication, transcription, and translation.

• DNA replication - the process of copying DNA before cell division

• Transcription - the process of creating RNA from DNA

• Translation - the process of creating proteins from RNA

• Genetic engineering - manipulating DNA to create new organisms or modify existing ones

• Gene expression - the process of turning genes on or off

• PCR - Polymerase Chain Reaction, a technique used to amplify DNA

• Sequencing...read more

PCR is a technique used to amplify DNA sequences. Quantification is the measurement of the amount of DNA present in a sample.

• PCR stands for Polymerase Chain Reaction

• It is used to make multiple copies of a specific DNA sequence

• Quantification can be done using methods like spectrophotometry or fluorometry

• It is important for determining the concentration of DNA in a sample

• PCR and quantification are commonly used in molecular biology research

DNA extraction is the process of isolating DNA from cells or tissues for further analysis.

• The first step is to break open the cells and release the DNA.

• This is usually done by adding a lysis buffer containing detergents and enzymes.

• The DNA is then separated from other cellular components using techniques such as centrifugation or filtration.

• Finally, the DNA is purified using alcohol precipitation or column chromatography.

• Common sources of DNA include blood, saliva, and tissue...read more