Vasudha Pharma Chem Junior Research Associate Interview Questions and Answers

HPLC analysis is a technique used to separate, identify, and quantify components in a mixture based on their interactions with a stationary phase and a mobile phase.

• HPLC stands for High Performance Liquid Chromatography

• It is commonly used in pharmaceutical, environmental, and food industries

• HPLC analysis involves injecting a sample into a column packed with a stationary phase, then eluting it with a mobile phase

• The com...read more

GC is a technique used for separating and analyzing compounds in a mixture, with various instrumentation and carrier gases. GCMS, DSC, NMR, and LCMS are related techniques.

• Gas Chromatography (GC) is a technique used to separate and analyze compounds in a mixture based on their interaction with a stationary phase and a mobile phase.

• Common carrier gases used in GC include helium, nitrogen, and hydrogen.

• GC instrumentation...read more

I would review the method development process, identify potential errors or issues, and troubleshoot to determine the cause of the discrepancy.

• Review the method development process to ensure all steps were followed correctly

• Check for any potential errors in the experimental setup or data analysis

• Verify the accuracy of the instruments and equipment used

• Consult with colleagues or experts for input and advice

• Perform addit...read more

Polarity refers to the separation of electric charge within a molecule, which affects its chemical properties.

• Polarity is determined by the difference in electronegativity between atoms in a molecule.

• Polar molecules have a positive and negative end, while nonpolar molecules have an even distribution of charge.

• Polarity influences various properties of molecules, such as solubility, boiling point, and intermolecular forc...read more

Chromatography is a technique used to separate and analyze mixtures of substances based on their different interactions with a stationary phase and a mobile phase.

• Gas chromatography (GC) separates volatile compounds based on their vapor pressure and affinity for the stationary phase.

• Liquid chromatography (LC) separates compounds based on their polarity and affinity for the stationary phase.

• Ion exchange chromatography s...read more

Chiral compounds are molecules that have a non-superimposable mirror image.

• Chiral compounds have an asymmetric carbon atom, also known as a chiral center.

• They exist in two enantiomeric forms, which are mirror images of each other.

• Chiral compounds exhibit optical activity, meaning they rotate the plane of polarized light.

• They have different physical and chemical properties from their mirror images.

• Examples of chiral com...read more