Shantha Biotechnics Research Intern Interview Questions and Answers

Q1. Conversation reaction
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Personality test to analyse individual's working style and over all mindsets.

Q1. Domain skills and knowledge
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Q1. Salary and other requirements
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Q1. Tell us about your expertise in Molecular Biology techniques.
Ans. 
I have expertise in various molecular biology techniques including PCR, DNA sequencing, cloning, and gene expression analysis.
• Proficient in PCR (Polymerase Chain Reaction) for amplifying DNA sequences

• Skilled in DNA sequencing methods such as Sanger sequencing and next-generation sequencing

• Experienced in molecular cloning techniques for gene manipulation and expression studies

• Knowledgeable in gene expression analysis us...read more
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Q2. What all cell culture techniques you are aware of?
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I am aware of various cell culture techniques including adherent cell culture, suspension cell culture, and 3D cell culture.
• Adherent cell culture involves cells growing attached to a surface, such as a petri dish or flask.

• Suspension cell culture involves cells growing freely in a liquid medium, without attachment to a surface.

• 3D cell culture involves growing cells in a three-dimensional environment to better mimic the ...read more
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Q3. What is your salary expectation?
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Q1. When can you join?
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Q2. Have you handled a multidisciplinary team before?
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Q1. How to handle and quench pyrophoric chemical in reaction.
Ans. 
Pyrophoric chemicals can be handled and quenched by using inert gases, specialized containers, and proper disposal methods.
• Use inert gases such as nitrogen or argon to displace air and prevent ignition.

• Store and handle pyrophoric chemicals in specialized containers such as air-tight glass ampoules or sealed metal containers.

• Dispose of pyrophoric chemicals properly by contacting a hazardous waste disposal company.

• Exampl...read more
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Q2. Name reactions based on palladium chemistry and copper chemistry
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Palladium and copper chemistry are widely used in organic synthesis. Some important reactions include Suzuki coupling, Heck reaction, Sonogashira coupling, and Ullmann reaction.
• Suzuki coupling: cross-coupling reaction between an organoboron compound and an organic halide or triflate catalyzed by a palladium complex

• Heck reaction: coupling of an aryl or vinyl halide with an alkene in the presence of a palladium catalyst

• S...read more
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Q3. Different oxidising and reducing agent for ester reduction,cyano reduction, alchol oxidation
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Different oxidising and reducing agents used for ester reduction, cyano reduction, and alcohol oxidation.
• Ester reduction: Lithium aluminum hydride (LiAlH4), sodium borohydride (NaBH4), diisobutylaluminum hydride (DIBAL-H)

• Cyano reduction: Lithium aluminum hydride (LiAlH4), sodium borohydride (NaBH4)

• Alcohol oxidation: Chromium trioxide (CrO3), potassium permanganate (KMnO4), Jones reagent (CrO3/H2SO4)
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Q4. Indole, oxazole and isoxazole synthesis route
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Q1. Organic chemistry conversion, reactions and mechanism, spectroscopy
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Q1. Tell me about yourself?
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Q2. How many years of experience do you have in contract research?
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Q3. What kind of skill you know?
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Q1. Tell me the name of any 5 oxidising reagents.??
Ans. 
Some common oxidising reagents include potassium permanganate, hydrogen peroxide, chromic acid, ozone, and nitric acid.
• Potassium permanganate

• Hydrogen peroxide

• Chromic acid

• Ozone

• Nitric acid
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Q1. Tell me your future goal??
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